A molecule breeding method for improving heat resistance of rice heading and flowering stage and filling stage by utilizing single-segment substitution line polymerization

A technology of heading and flowering stage and molecular breeding, which is applied in the fields of plant genetic improvement, botany equipment and methods, biochemical equipment and methods, etc. It can solve the problems of chain-linking and cumbersome hybrid offspring, limiting the cultivation of heat-resistant varieties, and not being easy to stabilize. To achieve the effect of long breeding cycle

Inactive Publication Date: 2018-10-26
JIANGXI ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

Breeders have always wanted to introduce the heat-resistant genes in Yuanjiang wild rice into cultivated rice through interspecific hybridization, but the breeding of heat-resistant varieties has been limited due to linkage cumbersomeness and the difficulty of stabilizing hybrid offspring.

Method used

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  • A molecule breeding method for improving heat resistance of rice heading and flowering stage and filling stage by utilizing single-segment substitution line polymerization
  • A molecule breeding method for improving heat resistance of rice heading and flowering stage and filling stage by utilizing single-segment substitution line polymerization
  • A molecule breeding method for improving heat resistance of rice heading and flowering stage and filling stage by utilizing single-segment substitution line polymerization

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Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1 selects parents

[0022] The Super Rice Research and Development Center of the National Rice Engineering Laboratory (Nanchang) of the Jiangxi Academy of Agricultural Sciences used Shuhui 527 as the recurrent parent and Yuanjiang wild rice (Hehuatang No. 3) as the donor parent. SSR molecular marker-assisted selection covering the whole genome has bred a set of 112 single-segment replacement lines of Yuanjiang common wild rice with the background of Shuhui 527, and the genome coverage rate reached 73.5%. In 2010, on the first day after rice heading, plants with consistent growth were selected and put into pots and moved into artificial climate chambers. Three plants were planted in each pot, and three replicates were set up for each material, and the water and fertilizer were managed conventionally. Set the relative humidity at 85%, light at 12h / d, and constant temperature at 38.0°C (day) / 28°C (night). After 5 days of treatment, the test materials were move...

Embodiment 2

[0025] (1) In the summer of 2011, a row of YJ07-04-06 and 9311 were planted at Nanchang Experimental Station of Jiangxi Academy of Agricultural Sciences, 9311 (CGMCC NO.12532) was the female parent, YJ07-04-06 (CGMCC NO.12534) and YJ01 -05-03 (CGMCCNO.12535) hybrid F 1 Formulate hybrid combinations for male parents to produce F 1 , all mixed harvest after maturity. Planting 3 rows of F in Sanya, Hainan in the winter of 2011 1 , backcross to obtain BC 1 f 1 , all mixed harvest after maturity. Field management was carried out by conventional methods.

[0026] (2) Molecular marker-assisted selection: 50 rows of BC were planted at the Nanchang Experimental Station of Jiangxi Academy of Agricultural Sciences in the summer of 2012 2 f 1 , 8 plants in each row, the collected leaves of each plant were put into 2 ml eppendorf tubes, filled with small steel balls, and then added 1.25% SDS extract solution 600 mu l. DNA was extracted by SDS method, and the molecular markers (Tab...

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Abstract

The invention belongs to the field of molecule breeding and relates to a molecule breeding method for cultivating rice with heat resistance which is increased heading and flowering stage and grain filling stage. The method includes the following steps: a middle indica rice two-line elite restorer lines restorer 9311 with the accession number CGMCC No.12532 is served as female parent, single-segment substitution lines, YJ-07-04-06 and YJ01-05-03, of Yuanjiang common wild rice are hybridized to obtain a F1 as parent, the female parent and the parent are intercrossed for one generation, backcrossed is performed for three generations with the recurrent parent, and then self-cross is performed for three generations to obtain the stable homozygous plant line BC3F4, and each generation is harvested with single spike; and the new rice line is identified and obtained by the molecule mark auxiliary selection combined with the phytotron simulation high temperature stress. Yuan ye no.3 with substantial heat resistance of the rice heading flowering stage and grain filling stage is cultivated by utilizing the method. The character of the target is selected through the molecule mark auxiliary, and the heat resistance restorer of the heading and flowering stage and grain filling stage can be rapidly and efficiently cultivated in 3-4 years.

Description

technical field [0001] The invention belongs to the field of molecular breeding, and relates to a molecular breeding method for improving rice heading and flowering stage and grain filling stage heat resistance by polymerization of single-segment substitution lines, in particular to a synchronous improvement of rice heading and flowering stage by polymerization of single-segment substitution lines of wild rice and molecular breeding methods for heat tolerance at the filling stage. Background technique [0002] High temperature heat damage is one of the main factors affecting the yield and quality of rice in the world, and it is also the main natural disaster of rice cultivation in China, which mainly occurs in the south of the Yangtze River Basin (Xiong Zhenmin, edited by Cai Hongfa. China Rice. Beijing: China Agricultural Science and Technology Press, 1992 ). High temperature disturbance in rice has the characteristics of sudden occurrence, large disaster area, and severe ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04C12Q1/6895
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172
Inventor 曹志斌袁林峰蔡耀辉聂元元李永辉谢红卫
Owner JIANGXI ACAD OF AGRI SCI
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