Molecular breeding method for improving rice heading and flowering period heat resistance by using single fragment substitution line

A technology of heading and flowering and molecular breeding, which is applied in horticultural methods, plant genetic improvement, botanical equipment and methods, etc., can solve the problems of interlocking redundant hybrid offspring, restricting the cultivation of heat-resistant varieties, and not easy to stabilize, and achieve a breeding cycle long effect

Inactive Publication Date: 2018-10-09
JIANGXI ACAD OF AGRI SCI
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Problems solved by technology

Breeders have always wanted to introduce the heat-resistant genes in Yuanjiang wild rice into cultivated rice through interspecific hybridization, but the breeding of heat-resistant varieties has been limited due to linkage cumbersomeness and the difficulty of stabilizing hybrid offspring.

Method used

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  • Molecular breeding method for improving rice heading and flowering period heat resistance by using single fragment substitution line
  • Molecular breeding method for improving rice heading and flowering period heat resistance by using single fragment substitution line
  • Molecular breeding method for improving rice heading and flowering period heat resistance by using single fragment substitution line

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Embodiment 1

[0020] Embodiment 1 selects parents

[0021]Jiangxi Provincial Academy of Agricultural Sciences Rice National Engineering Laboratory (Nanchang) Super Rice Research and Development Center used Shuhui 527 as the recurrent parent and Yuanjiang Wild Rice Hehuatang 3 as the donor parent (non-recurrent parent), self-crossed in the 5th generation of backcross Combining the third generation with SSR molecular marker-assisted selection covering the whole genome, a set of 112 single-segment replacement lines of Yuanjiang common wild rice with the background of Shuhui 527 were bred, and the genome coverage rate reached 73.5%. In 2010, on the first day after rice heading, plants with consistent growth were selected and placed in pots and moved into artificial climate chambers. Three plants were planted in each pot, and three replicates were set up for each material, and the water and fertilizer were managed conventionally. The relative humidity is set at 85%, the light is 12h / d, and the ...

Embodiment 2

[0023] (1) In the summer of 2011, a row of YJ07-04-06 and 9311 were planted at Nanchang Experimental Station of Jiangxi Academy of Agricultural Sciences, 9311 (CGMCC NO.12532) was the female parent, and YJ07-04-06 (CGMCC NO.12534) was the father F 1 , all mixed harvest after maturity. Planting 3 rows of F in Sanya, Hainan in the winter of 2011 1 , backcross to obtain BC 1 f 1 , all mixed harvest after maturity. Field management was carried out by conventional methods.

[0024] (2) Molecular marker-assisted selection: 50 rows of BC were planted at the Nanchang Experimental Station of Jiangxi Academy of Agricultural Sciences in the summer of 2012 2 f 1 , 8 plants in each row, the collected leaves of each plant were put into 2 ml eppendorf tubes, filled with small steel balls, and then added 1.25% SDS extract solution 600 mu l. DNA was extracted by SDS method, and the molecular markers (Table 1) on the imported fragments of YJ07-04-06 (CGMCC NO.5574) and 9311 (CGMCC NO.55...

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Abstract

The invention belongs to the field of molecular breeding, and relates to a molecular breeding method for improving rice heading and flowering period heat resistance by using a single fragment substitution line. According to the method, mid season indica rice two-line backbone restorer line 9311 with the preservation number being CGMCC NO. 12532 is used as a female parent; the wild rice chromosomefragment introgression line YJ07-04-06 with the preservation number being CGMCC NO. 12534 is used as a male parent; hybridization is performed for one generation; rotation crossing is performed threegenerations; then, self crossing is performed for three generations to obtain BC3F4; single plant harvesting is performed in each generation; then, molecular marker auxiliary selection is combined with an artificial climate room to simulate high temperature stress identification so as to obtain rice new strains. By using the method, the rice heading and flowering period heat resistance new strainof 'No. 2 Yuanye' is cultured. The target properties are selected in an auxiliary way through molecular markers; the heading and flowering period heat resistance restoration line can be fast and efficiently cultured in 3 to 4 years.

Description

technical field [0001] The invention belongs to the field of molecular breeding, and relates to a molecular breeding method for improving the heat resistance of rice heading and flowering stage by using a single-segment replacement line, in particular to a molecular breeding method for improving the heat resistance of rice heading and flowering stage by using a single-segment replacement line of wild rice method. Background technique [0002] High temperature heat damage is one of the main factors affecting the yield and quality of rice in the world, and it is also the main natural disaster of rice cultivation in China, which mainly occurs in the south of the Yangtze River Basin (Xiong Zhenmin, edited by Cai Hongfa. China Rice. Beijing: China Agricultural Science and Technology Press, 1992 ). High temperature disorder at the heading stage of rice (also known as heat damage) refers to the high temperature stress above 35°C during the heading and flowering period of rice, whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01G7/06C12Q1/6895
CPCA01G7/06A01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 曹志斌蔡耀辉聂元元毛凌华李永辉谢红卫
Owner JIANGXI ACAD OF AGRI SCI
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