Methods of immunogenic modulation

Abstract

Ex vivo determination of increased tumor immunogenicity of a tumor biopsy is used as a guide to identify immunotherapy of a tumor in a patient. Most preferably, the ex vivo tests will include exposureof biopsy samples to stress conditions to produce pretreated tumor cells that are then assayed with immune competent cells for increased activation or activity. Test conditions include exposure of the biopsy samples to immune stimulatory compositions, antibodies against neoepitopes, and / or modified cells, and an increase of immunogenicity is preferably determined by their exposure to T cells and / or NK cells.

Description

[0001] This application claims priority to US Provisional Application Serial No. 62 / 297,751, filed February 19, 2016. technical field [0002] The field of the invention is compositions and methods for treating cancer ex vivo to identify patient treatment options that increase the immunogenicity of the cancer in vivo. Background technique [0003] The Background Description includes information useful for understanding the present invention. It is not an admission that any information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art. [0004] All publications herein are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference. If the definition or usage of a term in an incorporated reference is inconsistent or contrary to the definition of that ter...

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Problems solved by technology

[0009] However, no one has taken into account that the expression of neoepitopes and other markers on cancer cells may vary according to the stress conditions given to the cancer cells, thus failing to identify their variation between patients

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