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Fish blood agar medium and preparation method thereof

An agar medium and culture medium technology, applied in bacteria and other directions, can solve the problems of isolation and identification of sudden diseases in aquaculture, long purchase cycle, short shelf life, etc. , low cost, good applicability

Inactive Publication Date: 2018-10-16
杭州富阳广华水产专业合作社 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the shelf life of this blood agar medium is very short, the cost is high, and the purchase cycle is long, which cannot well meet the isolation and identification needs of sudden diseases in aquaculture.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: culture medium preparation

[0017] Aseptically extract the blood of healthy grass carp and put it into a glass bottle filled with glass beads. Note that the glass beads are put into the glass bottle to sterilize and dry under high pressure, and then shake repeatedly for about 10-20 minutes until you see the glass beads covered with The fibrin can be stopped, and then divided into ultra-clean workbenches, and stored in a refrigerator at 2-8°C for later use. Generally, it can be stored for 2 weeks to one month. If hemolysis is found, it means that the fish blood cannot be used any longer.

[0018] The blood agar medium formula provided in this example contains peptone, beef extract powder, yeast powder, sodium chloride, defibrated fish blood and agar. Each 1000ml medium contains 15g of peptone, 5g of beef extract powder, 5g of yeast powder, 5g of sodium chloride, 50ml of defibrated fish blood and 15g of agar, with a pH range of 7.2±0.2.

[0019] The prepa...

Embodiment 2

[0020] Embodiment 2: Identification and isolation of pathogenic bacteria

[0021] Diseased grass carp were mainly collected from aquaculture farms in Hangzhou. Collect typical diseased grass carp, disinfect the surface, and take aseptically taken tissue samples of liver, kidney, intestine and other lesions, mark on sheep blood agar plate or fish blood plate, culture overnight at 30°C±2°C, and pick out suspicious colonies for pure culture . From the results of the culture experiment, the number of bacteria detected and the state of the fish blood plate were better than that of the sheep blood agar plate. A suspected pathogenic bacterium with typical β-type hemolysis was isolated and purified from the fish blood plate culture experiment, and it was recorded as SCSQ1415.

[0022] Gram staining showed that SCSQ1415 was Gram-negative bacteria. Aseptic operation scraping appropriate amount of bacteria, filling GN + Card, on-board identification. Physiological and biochemical an...

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PUM

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Abstract

The invention provides a fish blood agar medium. The fish blood agar medium comprises peptone, beef extract powder, sodium chloride, defibrinated fish blood and agar, wherein each 1000 ml of the medium contains 5-20 g of peptone, 1-10 g of beef extract powder, 1 to 10 g of yeast powder, 1 to 10 g of sodium chloride, 40 to 100 ml of defibrated fish blood and 10 to 20 g of agar, and the pH value ofthe medium is in a range of 7.0 to 8.0. The medium prepared in the invention has low cost, is simple in preparation procedure, can be stably used for the isolation and identification of sudden aquaticpathogenic bacteria, and presents better applicability than commercially available sheep blood agar mediums.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a fish blood agar medium formula, which is suitable for cultivating and screening fish pathogenic bacteria. Background technique [0002] At present, the commonly used method for isolation and identification of pathogenic microorganisms is to collect typical diseased fish, disinfect the surface, take liver, kidney, intestine and other lesion tissue samples aseptically, streak on nutrient agar plate or blood agar plate, and culture overnight at 30°C±2°C Afterwards, pick suspicious colonies for pure culture. Blood agar plate is a beef extract peptone medium containing defibrated animal blood (generally rabbit blood or sheep blood), so in addition to various nutrients needed for cultivating bacteria, it can also provide coenzyme (such as V factor), hemoglobin Special growth factors such as factor X. Blood agar medium is often used to isolate certain pathogenic microorganisms that are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 许宝青张广华戴瑜来童朝明冯晓宇
Owner 杭州富阳广华水产专业合作社
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