Nucleic acid amplification testing microfluidic chip and detection method thereof

Abstract

The invention discloses a nucleic acid amplification testing microfluidic chip and a detection method. The nucleic acid amplification testing microfluidic chip comprises a chip body, wherein the chipbody is provided with a sample feeding opening, an amplification reaction chamber, a diluting solution chamber, an immunochromatography chamber and a sealing plug, wherein a first soft membrane valveis arranged between the amplification reaction chamber and the diluting solution chamber; a second soft membrane valve is arranged between the amplification reaction chamber and the immunochromatography chamber; the diluting solution chamber is communicated with the amplification reaction chamber through the first soft membrane valve; the amplification reaction chamber is communicated with the immunochromatography chamber through the second soft membrane valve and a second flow channel; meanwhile, the amplification reaction chamber is communicated with the sample feeding opening. The soft membrane valves are used for blocking microfluidic and each chamber; meanwhile, the soft membrane valves can be broken by utilizing a mechanical force and then liquid mixing and reaction are carried out;nucleic acid amplification and immunochromatography technologies are designed on one microfluidic chip, so that the specificity and sensitivity of a nucleic acid diagnosis reagent are increased; complicated instruments used in a nucleic acid diagnosis process are reduced and the whole chip is completely sealed; pollution, caused by a nucleic acid amplification reagent, on the environment is effectively prevented.

Description

technical field [0001] The invention belongs to the technical field of medical equipment and relates to the field of microfluidic technology, in particular to a nucleic acid amplification detection microfluidic chip and a detection method thereof. Background technique [0002] Microfluidic chip (microfluidic chip) is currently a hot field in the development of Miniaturized Total Analysis Systems. The microfluidic chip analysis uses the chip as the operating platform, and at the same time is based on analytical chemistry, relying on micro-electromechanical processing technology, with micro-pipeline network as the structural feature, and life science as the main application object at present, with controllable micro-fluid throughout. A technology that systematically and completes various biological and chemical processes is the focus of development in the field of micro-total analysis systems. Its goal is to integrate the functions of the entire laboratory, including sampling...

AI Technical Summary

Problems solved by technology

However, the above-mentioned technical solutions fail to take into account immunochromatographic detection. As a stable and practical technology, nucleic acid amplification detection technology has been applied in various project detections. It requires specific nucleic acid extraction devices and detection instruments for extraction and amplification detection. , the reagents are liquid reagents, transportation and storage require cold chain transportation, and the cost is high; at the same time, nucleic acid detection is easy to pollute the environment, takes a long time, and the operation is cumbersome; immunological lateral flow diagnostic technology is suitable for instant detection as a stable and practical technology or on-site use

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