Three-dimensional high-speed wide view field tomography method and device thereof

Abstract

The invention provides a three-dimensional high-speed wide view field tomography method and a device thereof. The method comprises the following steps: 1, generating light beams; 2, performing high-speed space-time focusing surface scanning: performing dispersion and parallel collimation on the light beams, applying different phases to the light beams with different wavelengths / frequencies to carry out modulation in order to make the light beams have different time delays, and converging the modulated light beams onto a sample in order to form a focusing surface; 3, performing expanded field depth detection: arranging a high-speed zoom lens on the conjugate plane of the pupil entrance surface of an objective lens for lights formed by exiting the sample to achieve excitation end axial scanning synchronized imaging of object planes with different depths; and 4, simultaneously controlling: simultaneously controlling the light velocity modulation in the high-speed space-time focusing surface scanning step and the high-speed zoom lens in the developed field depth detection step. The method can improve the imaging speed and realize the high-time resolution three-dimensional imaging underthe premise of ensuring the spatial resolution.

Description

technical field [0001] The invention relates to the field of optical microscopy technology, in particular to a three-dimensional high-speed wide-field tomographic imaging method and device. Background technique [0002] Optical microscopic imaging technology is a technology to obtain high-resolution images of objects through optical methods. It is widely used in structural imaging and functional signal detection of microscopic objects, and has become a common method in biological research. In the existing microscopic imaging technology, ordinary wide-field single-photon imaging has a small penetration depth and does not have tomographic capabilities; although light-sheet imaging can achieve tomographic imaging, it cannot be applied to samples with strong scattering media. Multi-photon point-scanning imaging technology uses nonlinear optical effects to excite local signals, and has strong tomographic capabilities. In addition, the multiphoton point-scanning imaging technolog...

AI Technical Summary

Problems solved by technology

However, the above-mentioned pathways for changing the group velocity dispersion are constrained by mechanical inertia, which limits the speed of axial scanning

In addition, in order to achieve clear imaging, the image sensor (such as CCD, sCMOS, etc.) High-speed wide-field tomography still slow

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