Molecular marker for distinguishing Dengue fever and Dengue hemorrhagic fever

A technology for dengue hemorrhagic fever and dengue fever, which is applied in the field of molecular markers for distinguishing dengue fever and dengue hemorrhagic fever, can solve the problems of complex clinical manifestations of dengue virus infection and difficult to diagnose, and achieves good detection sensitivity and specificity. High volume and accurate results

Inactive Publication Date: 2018-09-28
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The clinical manifestations of dengue virus infection are very complex, and most of the symptoms are indistinguishable from diseases such as malaria, typhoid fever, and chikungunya fever. It is difficult to make a clear diagnosis based solely on the epidemiological data and clinical manifestations of patients, so laboratory tests are required for further diagnosis

Method used

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  • Molecular marker for distinguishing Dengue fever and Dengue hemorrhagic fever
  • Molecular marker for distinguishing Dengue fever and Dengue hemorrhagic fever
  • Molecular marker for distinguishing Dengue fever and Dengue hemorrhagic fever

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Screening of genes differentially expressed in patients with dengue fever and / or dengue hemorrhagic fever and normal people

[0046] 1. Research object:

[0047] Disease group: Collect 5 patients with dengue fever, 5 patients with dengue hemorrhagic fever, all patients according to "WHO / TDR.Dengue-Guidelines for diagnosis, Treatment, prevention and control.2009; http: / / www.who.int / tdr / publications / training-guideline-publications / dengue-diagnosis-treatment / en / index.html.Date of Access: 12 / 05 / 2015.2009" for diagnosis.

[0048] Normal group: select 4 cases of healthy volunteers.

[0049] There was no significant difference in age and gender between the two groups (P>0.10), which were comparable.

[0050] All research subjects were informed about this study and signed the informed consent.

[0051] 2. Extraction of total RNA in blood

[0052] (1) Fresh whole blood, erythrocyte lysate (1:1), mix by inverting several times, let stand for 5 minutes. 10000g, 4°C...

Embodiment 2

[0069] Example 2 Reverse transcription PCR experiment verifies the genes differentially expressed in patients with dengue fever and / or dengue hemorrhagic fever and normal people

[0070] The differentially expressed gene-CMIP gene screened out in Example 1 was selected for large sample verification.

[0071] 1. Research object:

[0072] Disease group: Collect 30 patients with dengue fever, 25 patients with dengue hemorrhagic fever, all patients according to "WHO / TDR.Dengue-Guidelines for diagnosis, Treatment, prevention and control.2009; http: / / www.who.int / tdr / publications / training-guideline-publications / dengue-diagnosis-treatment / en / index.html.Date of Access: 12 / 05 / 2015.2009" for diagnosis.

[0073] Normal group: select 30 cases of healthy volunteers.

[0074] There was no significant difference in age and gender between the two groups (P>0.10), which were comparable.

[0075] All research subjects were informed about this study and signed the informed consent.

[0076] 2....

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Abstract

The invention discloses a CMIP gene capable of being used as a molecular marker for distinguishing Dengue fever and Dengue hemorrhagic fever. By a high-throughput sequencing and RT-PCR method, the CMIP gene has significant expression difference in blood of normal people and patients with Dengue fever and Dengue hemorrhagic fever, i.e., whether a subject suffers the Dengue fever or the Dengue hemorrhagic fever can be judged by detecting the expression condition of the CMIP gene in the blood. According to the correlation of the Dengue fever and the Dengue hemorrhagic fever, the invention develops a kit for diagnosing the Dengue fever and the Dengue hemorrhagic fever. The kit is used for performing the Dengue fever and Dengue hemorrhagic fever diagnosis by detecting the CMIP gene expression.The diagnosis kit can be used for early diagnosis of diseases and has wide application prospects in clinics.

Description

technical field [0001] The invention belongs to the field of epidemiological diagnosis and prevention, and relates to a molecular marker for distinguishing dengue fever and dengue hemorrhagic fever. Background technique [0002] Since the Antonine plague (Shrap R, Lotka A J.A Problem in AgeDistribution[J].Philosophical Magazine, 1911,21:35-438) in the 2nd century AD, infectious diseases have been endangering human health and brought huge impacts on social stability. Impact. In recent years, the incidence of dengue infectious disease has increased rapidly and has become a world public health problem, and it is recorded as a disease in Appendix II of the newly revised International Health Regulations (2005). Dengue fever is an acute infectious disease caused by dengue virus and transmitted by Aedes mosquitoes. At first, people named it joint fever and fracture fever according to its symptoms (Monath, T.P. Dengue: The risk to developed and developing countries: Proc. Nat. Aca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/686C12Q1/6851C12Q1/6841C12Q1/6869G01N33/68G01N33/569
CPCC12Q1/6841C12Q1/6851C12Q1/686C12Q1/6869C12Q1/701G01N33/56983G01N33/68G01N2333/185Y02A50/30
Inventor 贾宏毅向常娟
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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