Gene marker for early cervical cancer screening and product and application thereof

A gene marker, cervical cancer technology, applied in the application field of early cervical cancer screening, can solve the problems of low sensitivity, low accuracy, reduction of cervical cancer incidence and mortality, and achieve high sensitivity and specificity. , the results are accurate and reliable, and the effect of good diagnostic efficiency

Active Publication Date: 2021-12-03
THE SECOND HOSPITAL OF HEBEI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, cervical exfoliated cell detection has been the standard method for screening cervical cancer. The application of this method has reduced the incidence and mortality of cervical cancer to a certain extent. However, the traditional cervical exfoliated cell detection method has obvious limitations. characteristics, such as the Pap cytology screening method, which is based on the subjective interpretation of the morphological changes of the cervical sample, and the sample cells in the transformation zone must be fully collected during the sampling process
Getting the right test results is more difficult when the incidence of lesions is low, especially when screening with the Pap test in people who have been vaccinated against HPV
In addition, medical staff fatigue due to the highly repetitive nature of screening tasks may increase the incidence of false results
That is to say, the traditional cervical exfoliated cell detection method has the disadvantages of low accuracy, low sensitivity, strong subjectivity, and easy to cause misdiagnosis and missed diagnosis.
It can be seen that there is still a lack of reliable gene markers reflecting the occurrence and development of cervical cancer in clinical practice, as well as products related to the gene markers. Its application in the screening of early cervical cancer is crucial to the field

Method used

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  • Gene marker for early cervical cancer screening and product and application thereof
  • Gene marker for early cervical cancer screening and product and application thereof
  • Gene marker for early cervical cancer screening and product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Screening for differentially expressed genes in cervical cancer

[0059] 1. Data source

[0060] The data used in this study are all from the Gene Expression Omnibus (GEO) database, with "cervical cancer" as the search keyword, searching in the GEO database, excluding studies at the cell line or animal level, and single-sample studies After the research, two sets of data sets were included, namely GSE63514 and GSE39001;

[0061] Among them, GSE63514 comes from the GPL570 platform, which contains 28 tissue samples of cervical cancer patients and 24 normal tissue samples; There are 43 tissue samples of cervical cancer patients and 12 normal tissue samples, and the data from GPL6244 contains 19 tissue samples of cervical cancer patients and 5 normal tissue samples;

[0062] The above-mentioned dataset GSE63514 downloaded from the GEO database is used as a training set, and the sample size is Case: Normal=28:24; the above-mentioned dataset GSE39001 downloaded fr...

Embodiment 2

[0073] Example 2 Verification of gene SPRR2B and CLU diagnostic efficacy

[0074] 1. Experimental method

[0075] For the genes SPRR2B and CLU differentially expressed in cervical cancer screened in Example 1, use the R package "pROC" (version 1.15.0) to perform receiver operating characteristic (ROC) analysis, and calculate the area under the curve (AUC) to To evaluate the accuracy, sensitivity and specificity of gene SPRR2B, gene CLU, and gene SPRR2B+CLU combination in the training set and validation set for the diagnosis of cervical cancer, respectively. AUC values ​​range from 0 to 1, where 0.7 is acceptable performance and 0.9 is excellent performance;

[0076] When judging the diagnostic efficacy of individual indicators in the training set and verification set, the expression of the gene is directly used for analysis, and the level corresponding to the point with the largest Youden index is selected as its cutoff value, and the genes with AUC of 0.5

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Abstract

The invention discloses a gene marker for early-stage cervical cancer screening as well as a product and application thereof, the gene marker is a combination of genes SPRR2B and CLU, verification finds that the gene marker has relatively good diagnostic efficiency, relatively high accuracy, sensitivity and specificity on cervical cancer, and in addition, the invention further provides a product for early screening of cervical cancer, and the product has important significance on early screening and early intervention treatment of cervical cancer and improvement of survival and living quality of patients.

Description

technical field [0001] The invention belongs to the field of in vitro diagnosis, in particular to gene markers SPRR2B and CLU for early cervical cancer screening, products related to the gene markers, and applications of the products in early cervical cancer screening. Background technique [0002] Cervical cancer is the second most common malignancy in developing countries and ranks the third among the causes of female cancer death (Schiffman M,Solomon D. Clinical practice.Cervical-cancer screening with human papillomavirus and cytologic competing[J].N Engl J Med.2013,369(24):2324-31.), in recent years, the global incidence of cervical cancer has shown a "younger" trend, and nearly 90% of cervical cancer deaths occurred in developing countries. Regional differences in cervical cancer incidence reflect differences in the effectiveness of cervical cancer screening (used to detect and rule out cervical precancer) and in rates of human papillomavirus (HPV) infection. Human pap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574G16B5/00G16B25/10G16B30/00G16B35/20G16B50/00
CPCC12Q1/6886G01N33/57411G01N33/57484G16B5/00G16B25/10G16B30/00G16B35/20G16B50/00C12Q2600/158G01N2333/47
Inventor 王文莉张保连刘宏
Owner THE SECOND HOSPITAL OF HEBEI MEDICAL UNIV
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