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Antibiotic degradation mixed bacterial agent and application thereof

A technology of mixing bacterial agents and antibiotics, applied in the directions of bacteria, fungi, microorganisms, etc., can solve the problem of few antibiotics degrading mixed bacterial agents, and achieve the effect of clear bacterial composition, obvious biodegradation advantages, and good application prospects.

Active Publication Date: 2018-09-18
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, there are still few reports on the definite antibiotic degradation of mixed bacterial agents by such bacteria.

Method used

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  • Antibiotic degradation mixed bacterial agent and application thereof

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Experimental program
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Effect test

Embodiment

[0039] 1. The domestication, screening and identification methods of Arthrobacter sp. and Alcaligenes sp. include the following steps: 1) Get about 5g of sludge and add the target antibiotic (1, oxytetracycline, 50mg / L; 2) respectively , sulfadimethoxine, 50 mg / L) nutrient-poor enrichment medium and nutrient-enrichment medium, and placed in a constant temperature shaker (30°C, 150-180rpm) for one week in the dark.

[0040] 2) After the culture of the previous stage was allowed to stand, 5 ml of the supernatant was taken and transferred to the same resistance-enriched medium. After culturing for 7 days, the residual concentration of antibiotics was measured. A total of 3 cycles of enrichment were performed.

[0041] 3) Compare the antibiotic residue concentrations in the oligotrophic enrichment culture and the eutrophic enrichment culture, select the enrichment culture with a higher antibiotic degradation rate, and after standing for 5-10min, take 0.2mL of the culture and sprea...

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Abstract

The invention relates to the field of environmental protection, and discloses an antibiotic degradation mixed bacterial agent and application thereof. The mixed bacterial agent is prepared from Arthrobacter sp. and Alcaligenes sp.. The Arthrobacter sp. is tetracycline antibiotic degradation arthrobacterium, and can be used for simultaneously degrading oxytetracycline, tetracycline and aureomycin;the Alcaligenes sp. is sulfanilamide antibiotic degradation bacterium, can be used for degrading sulfadimethoxine, and can tolerate sulfadimidine, sulfadiazine and sulfamonomethoxine. The antibiotic degradation strains and bacterial agents can be used for degradation removal of residue antibiotics in waste such as livestock and poultry excrement; the release of the residue antibiotic in the wasteto the environment can be reduced; meanwhile, the bacterial agent can be applied to the degradation removal of the antibiotic contaminants in the antibiotic polluted soil; when the bacterial agent isused for environmental restoration, good application prospects and environmental benefits are realized.

Description

technical field [0001] The invention relates to the field of environmental protection, in particular to an antibiotic-degrading mixed bacterial agent and its application. Background technique [0002] Antibiotic residues are common in the environment, and antibiotics have been detected in soil, sewage sludge, surface water, and animal and plant foods, causing harm to the human body and the ecological environment. Numerous studies have shown that the detectable antibiotic concentration in environmental soil is close to the content level of other pesticide organic pollutants in soil. How to effectively remove antibiotic residues in the environment has become an important environmental problem to be solved urgently. [0003] Biodegradation is an effective means to solve the environmental residues of antibiotics, which has attracted extensive attention of scholars at home and abroad. Currently, tetracycline and sulfonamide antibiotic-degrading bacteria have been reported. Deg...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C02F3/34B09C1/10C12R1/06C12R1/05C12R1/645
CPCB09C1/10B09C1/105C02F3/34C02F3/347C12N1/14C12N1/20
Inventor 林辉马军伟张昕孙万春俞巧钢叶静王强邹平陈照明符建荣
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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