A kind of antibiotic degrading mixed bacteria agent and its application

A technology of mixing bacterial agents and antibiotics, applied in the directions of bacteria, fungi, microorganisms, etc., can solve the problem of few antibiotics degrading mixed bacterial agents, and achieve the effects of clear bacterial composition, high degradation efficiency, and good application prospects.

Active Publication Date: 2019-06-28
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, there are still few reports on the definite antibiotic degradation of mixed bacterial agents by such strains

Method used

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  • A kind of antibiotic degrading mixed bacteria agent and its application

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Embodiment

[0039] One, the domestication, screening and identification method of Arthrobacter sp. and Alcaligenes sp., comprising the following steps:

[0040] 1) Take about 5g of sludge and add them to the oligotrophic enrichment medium and nutrient enrichment medium containing target antibiotics (1, oxytetracycline, 50mg / L; 2, sulfadimethoxine, 50mg / L) respectively , placed in a constant temperature shaker (30° C., 150-180 rpm) and incubated in the dark for one week.

[0041] 2) After the culture of the previous stage was allowed to stand, 5 ml of the supernatant was taken and transferred to the same resistance-enriched medium. After culturing for 7 days, the residual concentration of antibiotics was measured. A total of 3 cycles of enrichment were performed.

[0042] 3) Compare the antibiotic residue concentrations in the oligotrophic enrichment culture and the eutrophic enrichment culture, select the enrichment culture with a higher antibiotic degradation rate, and after standing fo...

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Abstract

The invention relates to the field of environmental protection, and discloses an antibiotic-degrading mixed bacterial agent and its application. The mixed bacterial agent includes: Arthrobacter Arthrobacter sp. and Alcaligenes Alcaligenes sp. . Arthrobacter in the present invention Arthrobacter sp. It degrades Arthrobacter for tetracycline antibiotics and can simultaneously degrade oxytetracycline, tetracycline and aureomycin; Alcaligenes Alcaligenes sp. It is a sulfa antibiotic-degrading bacteria that can degrade sulfamethoxine and is resistant to sulfamethazine, sulfadiazine and sulfa-6-methoxine. The above antibiotic-degrading bacteria strains and bacterial agents can be used to degrade and remove residual antibiotics in waste such as livestock and poultry manure, reduce the release of residual antibiotics in waste to the environment, and can be applied to the degradation and removal of antibiotic pollutants in antibiotic-contaminated soil to improve environmental protection. Restoration has good application prospects and environmental benefits.

Description

technical field [0001] The invention relates to the field of environmental protection, in particular to an antibiotic-degrading mixed bacterial agent and its application. Background technique [0002] Antibiotic residues are common in the environment. Antibiotics have been detected in soil, sewage sludge, surface water, and animal and plant foods, causing harm to the human body and the ecological environment. Many studies have shown that the detectable concentration of antibiotics in environmental soil is close to the content level of other pesticide organic pollutants in soil. How to effectively remove antibiotic residues in the environment has become an important environmental problem to be solved urgently. [0003] Biodegradation is an effective means to solve the environmental residues of antibiotics, which has attracted extensive attention of scholars at home and abroad. Currently, tetracycline and sulfonamide antibiotic-degrading bacteria have been reported. Tetracy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C02F3/34B09C1/10C12R1/06C12R1/05C12R1/645
CPCB09C1/10B09C1/105C02F3/34C02F3/347C12N1/14C12N1/20
Inventor 林辉马军伟张昕孙万春俞巧钢叶静王强邹平陈照明符建荣
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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