Rhodococcus inoculant and remediation method of rhodococcus inoculant on organic chlorine polluted soil

A technology of organochlorine and bacterial agent, applied in the preparation method of bacterial agent and its bioremediation of organochlorine polluted environment, and in the field of bioremediation of organochlorine polluted environment in the environment, which can solve the problems of increasing operating costs and achieve clear bacterial composition , easy to prepare, and environmentally friendly

Pending Publication Date: 2022-07-29
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, sludge is usually produced during physical and chemical treatment processes such as adsorption, photolysis, and electrolysis, which need to be further treated in sanitary landfills, which in turn increases operating costs

Method used

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  • Rhodococcus inoculant and remediation method of rhodococcus inoculant on organic chlorine polluted soil
  • Rhodococcus inoculant and remediation method of rhodococcus inoculant on organic chlorine polluted soil
  • Rhodococcus inoculant and remediation method of rhodococcus inoculant on organic chlorine polluted soil

Examples

Experimental program
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Embodiment 1

[0022] Example 1. Strain screening

[0023] Materials and methods

[0024] Soil sample: Long-term organochlorine-contaminated soil near a pharmaceutical factory in Nanjing.

[0025] Inorganic salt medium: NaCl 0.5g, K 2 HPO 4 1.5 g, KH 2 PO 4 0.5 g, distilled water 1000mL, adjust pH to 7.2~7.5, sterilize at 121℃ for 20min before use.

[0026] Screening medium for p-chloroaniline degrading bacteria: add a certain amount of p-chloroaniline on the basis of inorganic salt medium.

[0027] Screening of p-chloroaniline-degrading strains

[0028] Weigh 4g of original soil into a 100mL conical flask containing organic pollutants (100mg / L). Place in a shaker with continuous shaking (180 rpm) in the dark at 30°C and incubate for 7 days.

[0029] After 7 days of incubation, the incubation was observed. After standing, 1 mL of the suspension was transferred to 100 mL of inorganic salt medium with organic pollutant concentrations of 200, 300, 400 and 500 mg / L.

[0030] The enrich...

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Abstract

The invention belongs to the technical field of bioremediation of parachloroaniline polluted environment, and particularly relates to a fungicide, a preparation method of the fungicide and application of the fungicide in the field of organochlorine polluted environment. The invention provides a p-chloroaniline degrading bacterium, which is Rhodococcus zopfii DCB-5 and is obtained by screening from organic chlorine polluted soil near a pharmaceutical factory in Nanjing. The microbial agent disclosed by the invention has a good degradation rate on parachloroaniline, and the prepared microbial agent has a good application prospect in the aspect of bioremediation of organic chlorine polluted environments of water bodies and soil.

Description

technical field [0001] The invention belongs to the technical field of bioremediation of organic chlorine-polluted environments in the environment, relates to a bacterial agent composed of Rhodococcus zopfii DCB-5, and specifically includes a preparation method of the bacterial agent and its application in the field of organic chlorine-polluted environmental bioremediation. application. Background technique [0002] With the increase in the use of organochlorine and related products in industry and agriculture, the damage to the environment caused by the compound pollution of organochlorine compounds cannot be ignored. Although the environment has a certain "self-purification" effect, it can degrade and transform some toxic organic pollutants, but in most cases, the natural decay process is very long and incomplete. There have been a lot of reports on the pollution incidents of organochlorine compounds in the aquatic environment, sediments and soil environments. Once they e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02B09C1/10C12R1/01
CPCC12N1/20C12N1/02B09C1/10
Inventor 孙烁徐晨菲乔晨露郭龙飞唐明璐李婧唐方赵朝成刘其友
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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