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Polyphenol coating for capturing circulating tumor cells

A tumor cell and coating technology, applied in the field of biofunctional materials and analysis and detection, can solve the problems of low capture efficiency, long enrichment time, complicated preparation process, etc., and achieve low cost, simple and fast method, and low price Effect

Inactive Publication Date: 2018-09-14
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have a certain capture effect on CTCs, there are still various shortcomings, such as the complicated material preparation process, some capture efficiency is not high or the enrichment time is long, etc.
In addition, since CTCs undergo epithelial-mesenchymal transition and not every tumor cell expresses epithelial markers, capturing CTCs with a single EpCAM antibody often leads to false-negative results
In addition, the production of antibodies is expensive, and the combined use of multiple specific marker antibodies will lead to an increase in cost; and the stability of nucleic acid aptamers is insufficient, and they are easily degraded by nucleases
There is no similar report on the use of polyphenolic compounds for CTCs capture

Method used

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  • Polyphenol coating for capturing circulating tumor cells
  • Polyphenol coating for capturing circulating tumor cells
  • Polyphenol coating for capturing circulating tumor cells

Examples

Experimental program
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Effect test

Embodiment 1

[0027] The polyphenol compound used in this example is tannic acid, and the base material is a flat single crystal silicon wafer; the base material is chemically modified by the chelation self-assembly of polyphenol compounds and metal ions; the cancer cell MCF-7 is used as Taking circulating tumor cells to be captured as an example, the capture system of the present invention is further described and verified, including the following steps:

[0028] 1) A polyphenol coating is prepared on the surface of the substrate silicon wafer by using a modification method of chelating self-assembly of polyphenol compounds and metal ions:

[0029] Prepare 3.2mg / mL tannic acid aqueous solution and 0.8mg / mL ferric chloride aqueous solution respectively; add 1mL tannic acid solution and 1mL ferric chloride solution to a 1cm×1cm substrate silicon wafer in sequence , reacted for 3 minutes, took out the silicon chip after the reaction was completed, washed with deionized water, and dried with n...

Embodiment 2

[0038] The polyphenol compound used in this example is epicatechin gallate, and the base material is a flat gold sheet; the base material is chemically modified by the chelation self-assembly of the polyphenol compound and metal ions; the cancer cell MCF- 7. Taking circulating tumor cells to be captured as an example, the capture system of the present invention is further described and verified, including the following steps:

[0039] 1) Using the modification method of polyphenolic compound and metal ion chelation self-assembly, prepare polyphenolic coating on the surface of substrate gold sheet:

[0040] Separately prepare epicatechin gallate aqueous solution with a concentration of 3.2mg / mL and ferric chloride aqueous solution with a concentration of 0.8mg / mL; add 1mL of epicatechin gallate solution to a 1cm×1cm base gold sheet in sequence React with 1mL ferric chloride solution for 3 minutes. After the reaction is completed, take out the gold flakes, wash them with deionized...

Embodiment 3

[0049] The polyphenolic compound selected in this embodiment is tannic acid, and the substrate material is polydimethylsiloxane (PDMS); the substrate material is chemically modified by the chelating self-assembly of the polyphenol compound and metal ions; The cells MCF-7, A431, Hela and A549 are taken as examples of circulating tumor cells to be captured, and the capture system of the present invention is further described and verified, including the following steps:

[0050] 1) Using the modification method of chelating self-assembly of polyphenolic compounds and metal ions, a polyphenolic coating is prepared on the surface of the substrate PDMS:

[0051] Prepare 3.2mg / mL tannic acid aqueous solution and 0.8mg / mL ferric chloride aqueous solution respectively; add 1mL tannic acid solution and 1mL ferric chloride solution to the 1cm×1cm PDMS substrate, React for 3 minutes. After the reaction is completed, take out the PDMS, wash with deionized water, and dry with nitrogen; repe...

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Abstract

The invention provides a polyphenol coating for capturing circulating tumor cells. A polyphenol type compound is modified on the surface of a material by adopting a surface modification method; the polyphenol coating can be used for rapidly and effectively enriching the circulating tumor cells. A capturing technology of the circulating tumor cells, provided by the invention, does not need to immobilize a specific cancer cell identification antibody or an aptamer, and specific capturing of different cancer cell types can be realized; a micro-nano base material does not need to be introduced, sothat the polyphenol coating has the advantages of low cost and convenience for operation. The prepared polyphenol coating can be used for capturing the circulating tumor cells in peripheral blood andis expected to be used for early diagnosis of patients with cancers.

Description

technical field [0001] The invention belongs to the technical field of biofunctional materials and analysis and detection, and particularly relates to the technology of capturing circulating tumor cells on the surface of materials. Background technique [0002] Cancer is one of the most serious diseases threatening human health. Tumor invasion and metastasis are the main causes of clinical death in cancer patients. Early detection and treatment are the most effective means to improve the survival rate of cancer patients. Circulating Tumor Cells (CTCs) are cells that break away from in situ tumor lesions and enter the human peripheral blood circulation system during the formation or growth of tumors. Studies have shown that changes in the number and type of CTCs in blood are closely related to the occurrence and development of cancer, and they are one of the most potential methods for non-invasive diagnosis of tumors and real-time efficacy monitoring. Therefore, the developm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
CPCC12N5/0693
Inventor 贾凌云杨立为韩璐璐任军
Owner DALIAN UNIV OF TECH
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