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Sperm gradient centrifugation liquid and preparation method thereof

A gradient centrifugation and sperm technology, applied in the field of assisted reproduction, can solve the problems of limited pH buffer capacity, large pH fluctuation, and inability to reduce physical damage, and achieve strong buffer capacity and reduce sperm damage.

Active Publication Date: 2018-09-14
成都艾伟孚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The buffer used in most sperm gradient centrifugation fluids is HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid). The pH buffering capacity of a single buffer system is limited, and the pH fluctuation is large; on the other hand, generally Sperm gradient centrifugation fluid relies on the addition of glucose to enable sperm capacitation, but does not reduce the physical damage to sperm caused by centrifugation

Method used

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  • Sperm gradient centrifugation liquid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Sperm Gradient Centrifugation Solution 3

[0058] 1) Using water as the base, prepare the base liquid according to the basic formula;

[0059] 2) Add C component 10mmol / L and D component 4.10mmol / L, divide the solution into 2 equal parts, marked as solution 1 and solution 2;

[0060] 3) Add component A to solution 1 to make the final concentration reach 40%, which is marked as a1, and add component A to solution 2 to make the final concentration reach 80%, which is marked as a2;

[0061] 4) a1 and a2 were sterilized by filter membrane respectively.

Embodiment 2

[0062] Example 2 Sperm Gradient Centrifugation Solution 4

[0063] 1) Using water as the base, prepare the base liquid according to the basic formula;

[0064] 2) Add C component 10mmol / L and D component 4.10mmol / L, add B component 30mg / L to divide the solution into 2 equal parts, marked as solution 1 and solution 2,

[0065] 3) Add component A to solution 1 to make the final concentration reach 40%, which is marked as a1, and add component A to solution 2 to make the final concentration reach 80%, which is marked as a2;

[0066] 4) a1 and a2 were sterilized by filter membrane respectively.

Embodiment 3

[0067] Example 3 Sperm Gradient Centrifugation Solution 5

[0068] 1) Using water as the base, prepare the base liquid according to the basic formula;

[0069] 2) Add C component 10mmol / L and D component 4.10mmol / L, add B component 100mg / L to divide the solution into 2 equal parts, marked as solution 1 and solution 2,

[0070] 3) Add component A to solution 1 to make the final concentration reach 40%, which is marked as a1, and add component A to solution 2 to make the final concentration reach 80%, which is marked as a2;

[0071] 4) a1 and a2 were sterilized by filter membrane respectively.

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Abstract

The invention belongs to the field of assisted reproduction, and particularly relates to sperm gradient centrifugation liquid and a preparation method thereof. According to the sperm gradient centrifugation liquid, pH (potential of hydrogen) of the sperm gradient centrifugation liquid is adjusted and controlled by HEPES+MOPS double-buffering systems, the pH is more stable, a key raw material lecithin is added into the sperm gradient centrifugation liquid, sperm activity is excited, and damage to sperms is repaired and reduced. Sperms selected by the sperm gradient centrifugation liquid are high in activity and higher in normal from rate, and full preparation is made for subsequent in-vitro fertilization.

Description

technical field [0001] The invention belongs to the field of assisted reproduction, and in particular relates to a sperm gradient centrifugal liquid and a preparation method thereof. Background technique [0002] In Vitro Fertilization (In Vitro Fertilization) refers to the technology in which human sperm and eggs complete the fertilization process in an artificially controlled environment outside the body. It is abbreviated as IVF in English. A large number of people are unable to conceive naturally due to physical conditions, which requires in vitro fertilization-embryo transfer, commonly known as test-tube baby technology, for reproduction. According to a survey data, the infertility rate in my country has climbed from 2.5%-3% 20 years ago to 12.5%-15%. The number of newborns born in my country is about 16 million per year. Based on the infertility rate of 12.5%-15%, in theory, 2-2.4 million newborns cannot be born each year due to infertility. With the implementation of...

Claims

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Application Information

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IPC IPC(8): C12N5/076
CPCC12N5/061C12N2509/10
Inventor 余裕炉米乐刘洪君严飞戴甄
Owner 成都艾伟孚生物科技有限公司
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