Micro-cavity type multicolor fluorescence digital PCR (Polymerase Chain Reaction) chip and application

Abstract

The invention relates to a micro-cavity type multicolor fluorescence digital PCR (Polymerase Chain Reaction) chip and application. The micro-cavity type multicolor fluorescence digital PCR chip comprises a liquid inlet, a double-side micro-cavity array and a double-side waste liquid cabin. The chip is used for carrying out trace cancer rare mutation analysis, differential genes expression analysisor high-precision copy number variation analysis. The micro-cavity type multicolor fluorescence digital PCR chip utilizes multicolor fluorescence detection and the detection flux of the single chip can be greatly improved; the reaction efficiency is extremely improved, the operation is convenient and the speed is rapid; the extremely high detection sensitivity can be realized and the micro-cavitytype multicolor fluorescence digital PCR chip has a good application prospect.

Description

technical field [0001] The invention belongs to the field of liquid biopsy, in particular to a microcavity multicolor fluorescent digital PCR chip and its application. Background technique [0002] In recent years, with the change of residents' lifestyle and factors such as environmental pollution, malignant tumors have become the biggest killer of residents' health. In 2012, approximately 14.1 million people were diagnosed with cancer and 8.2 million people died of cancer (equivalent to 14.6% of the total annual death toll). Due to the extremely complex etiology and pathogenesis of cancer, and individual differences in clinical manifestations, most patients have developed to an advanced stage when they are diagnosed. Clinical statistics show that, taking lung cancer as an example, its overall 5-year survival rate is only 10%-16%, while the 5-year survival rate of stage I patients can reach 65-75%. Therefore, early detection and early treatment have a positive effect on pr...

AI Technical Summary

Problems solved by technology

However, the current digital PCR system mainly has the following deficiencies: the integration of the system is low

QX200, Raindrop, and QuantStudio systems all need to use two to three different instruments to complete the steps of digital PCR sample dispersion, amplification, and detection, which are cumbersome and expensive.

During the transfer and switching of samples in these instruments, there is a risk of contamination, resulting in false positive signals

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