Clostridium butyricum fermentation method for obviously improving yield of butyric acid

Abstract

The invention discloses a clostridium butyricum fermentation method for obviously improving yield of butyric acid. The clostridium butyricum fermentation method for obviously improving yield of butyric acid comprises the following steps: S1, inoculating clostridium butyricum into a culture medium, culturing until the pH value of a culture solution is smaller than 3.5, performing tolerance treatment under the condition of a water bath of 80-85 DEG C at the early stage of clostridium butyricum sporulation, collecting bacterium liquid subjected to tolerance treatment, and separating tolerant strains; S2, inoculating the tolerant strains in the S1 into a liquid shake flask medium, and culturing to a logarithmic phase to obtain primary seeds; S3, taking the primary seeds in the S2 accounting for 1% of the total amount of the culture into a primary culture medium of a 50 L of seeding tank, and culturing to a logarithmic phase to obtain secondary seeds; and S4, inoculating the secondary seedsin the S3 accounting for 1.5% of the total amount of the culture into a secondary culture medium of a fermentation tank, culturing to the logarithmic phase, dropping a sodium carbonate solution intothe fermentation tank when the pH value of the culture solution is detected to be 5.5, and continuously culturing to the later stage of spores.

Description

technical field [0001] The invention relates to the field of butyric acid prepared by fermentation of Clostridium butyricum, in particular to a fermentation method of Clostridium butyricum which significantly increases the yield of butyric acid. Background technique [0002] For a long time, feed antibiotics have been effective in safeguarding animal health, promoting animal growth, and improving feed utilization, bringing huge economic benefits to livestock and poultry production. However, with the improvement of people's living standards and the strengthening of health awareness, problems such as drug resistance and drug residues caused by antibiotics in feed are increasingly paid attention to, so governments of various countries have introduced measures to strictly control the use of antibiotics as feed additives. Therefore, the development of antibiotic substitutes has become a hot spot in the research of feed additives. Short-chain fatty acids (SCFA) include acetic aci...

AI Technical Summary

Problems solved by technology

[0004] At present, the production of butyric acid by microbial fermentation is a hot spot in the research of biomass energy, but there are few studies on the extraction of butyric acid from fermentation broth.

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