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Rapid separation method of plasma of maternal peripheral blood and application of method

A separation method and peripheral blood technology, applied in biochemical equipment and methods, chemical libraries, combinatorial chemistry, etc., can solve the problems of increasing manpower and material costs, increasing sample confusion in plasma separation, and reducing rechecking actions and manpower The probability of confusion and the effect of reducing the secondary marking action

Inactive Publication Date: 2018-09-07
CAPITALBIO GENOMICS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the two-step centrifugation method involves a tube transfer operation in the middle, which increases the probability of sample confusion in the plasma separation process; and the centrifugation equipment needs to support low-speed centrifugation of blood collection tubes and high-speed centrifugation of centrifuge tubes. The above conditions increase manpower and material costs virtually.

Method used

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  • Rapid separation method of plasma of maternal peripheral blood and application of method
  • Rapid separation method of plasma of maternal peripheral blood and application of method
  • Rapid separation method of plasma of maternal peripheral blood and application of method

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Effect test

Embodiment 1

[0054] Embodiment 1, a kind of rapid separation method of maternal peripheral blood plasma

[0055] Collect 5 mL of maternal peripheral blood, and obtain plasma by one-step centrifugation. The specific operation of the one-step centrifugation is: put into a blood collection tube containing maternal peripheral blood, and centrifuge for 10 minutes with a relative centrifugal force of 1600 g. The supernatant is the obtained plasma. Store at -20°C for later use.

Embodiment 2

[0056] Embodiment 2, a kind of rapid separation method of maternal peripheral blood plasma

[0057] Collect 5mL of maternal peripheral blood, and obtain plasma by one-step centrifugation. The specific operation of the one-step centrifugation is: put into a blood collection tube filled with maternal peripheral blood, and centrifuge for 10 minutes with a relative centrifugal force of 2500g. The supernatant is the obtained plasma. Store at -20°C for later use.

Embodiment 3

[0058] Embodiment 3, a kind of rapid separation method of maternal peripheral blood plasma

[0059] Collect 5 mL of maternal peripheral blood, and obtain plasma by one-step centrifugation. The specific operation of the one-step centrifugation is: put into a blood collection tube containing maternal peripheral blood, centrifuge for 10 minutes with a relative centrifugal force of 3200 g, and the supernatant is the obtained plasma. Store at -20°C for later use.

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Abstract

The invention discloses a rapid separation method of plasma of maternal peripheral blood and an application of the method. The rapid plasma separation method comprises steps as follows: isolated maternal peripheral blood is further centrifuged, and plasma is obtained; compared with plasma treated with a two-step centrifugal method, the separated plasma has equivalent free DNA concentration, free DNA fragment distribution, fetal DNA concentration and sequencing fragment distribution after free DNA extraction, library construction, high-throughput sequencing and bio-information analysis, and canbe applied to free DNA extraction, fetal nucleic acid information extraction and noninvasive prenatal gene screening products. Cognition and practice of the two-step centrifugal method for conventional plasma separation are broken through, the operation procedure for plasma separation is simplified, labor and material resource input are reduced, and the method has good market application value.

Description

technical field [0001] The invention relates to the field of free nucleic acid sequencing, in particular to a method for rapid separation of maternal peripheral blood plasma and its application. Background technique [0002] In the 1940s, French scientists Mandel and Metais discovered that there is free extracellular DNA in peripheral blood, which is called peripheral blood free DNA (Circulating DNA in plasma or serum). In 1997, Lu Yuming and others detected the specific sequence of the Y chromosome in the peripheral blood of pregnant women with male fetuses, proving that there is free fetal DNA (cell-free fetal DNA, cffDNA) in the peripheral blood of pregnant women, and the discovery of cffDNA is a non-invasive birth. Pre-genetic screening (NIPT) of fetuses for birth defects has ushered in a new era. Compared with traditional invasive prenatal diagnosis (chorionic villus sampling, amniocentesis and umbilical cord blood puncture), non-invasive prenatal genetic screening has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12Q1/6806C12Q1/6869C40B50/06
CPCC12N15/1013C12Q1/6806C12Q1/6869C40B50/06C12Q2535/122
Inventor 糜庆丰罗东红陈样宜谢婵芳黄铨飞饶兴蔷彭春方
Owner CAPITALBIO GENOMICS
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