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Method for extracting polysaccharide of dendrobium officinale

A technology for Dendrobium officinale polysaccharide and Dendrobium officinale, which is applied in the field of extracting Dendrobium officinale polysaccharide and achieves the effects of high extraction rate, low volatility and mild reaction conditions

Active Publication Date: 2018-09-07
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It can be seen that there is still a lack of technology that combines deep eutectic solvent and enzyme extraction to further extract polysaccharides efficiently.

Method used

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  • Method for extracting polysaccharide of dendrobium officinale
  • Method for extracting polysaccharide of dendrobium officinale
  • Method for extracting polysaccharide of dendrobium officinale

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Mix the Dendrobium officinale powder with the deep eutectic solvent (choline chloride: glycerol=1:2, mol / mol) according to the solid-liquid ratio of 1g: 30mL, then add the compound enzyme ( Cellulase: pectinase=1:1, mg / mg), extracted at 50°C for 90min;

[0029] (2) The obtained extract is subjected to solid-liquid separation, and the supernatant is purified with macroporous resin AB-8 to obtain crude sugar liquid;

[0030] (3) Then use a dialysis bag (3500Da) to dialyze the crude sugar solution in running water for 24 hours, then dialyze it in distilled water for 24 hours, change the distilled water every 8 hours, and then vacuum concentrate it to 1 / 4 of the original volume at 50°C. Add 95% ethanol solution equivalent to 4 times the volume of the concentrated solution to precipitate the polysaccharide. Specifically, during the precipitation process, add slowly while stirring, first add an ethanol solution equal to the concentrated solution to precipitate the polysac...

Embodiment 2

[0037] (1) Mix the Dendrobium officinale powder with the deep eutectic solvent (choline chloride: glycerol=1:6, mol / mol) according to the solid-liquid ratio of 1g: 50mL, then add the compound enzyme ( Cellulase: pectinase=1:1, mg / mg), extract at 55°C for 100min;

[0038] Steps (2)-(4) refer to Example 1.

[0039] The extraction rate of polysaccharide was determined to be 45%, the recovery rate of polysaccharide separated and purified by AB-8 macroporous resin was 96%, the decolorization rate and protein removal rate were 98% and 98% respectively, and the purity of the final purified polysaccharide was 98%.

Embodiment 3

[0041] (1) Mix the Dendrobium officinale powder with the deep eutectic solvent (choline chloride: glycerol=1:2, mol / mol) according to the solid-liquid ratio of 1g: 30mL, then add the compound enzyme ( Cellulase: pectinase=1:1, mg / mg), extracted at 30°C for 120min;

[0042] Steps (2)-(4) refer to Example 1.

[0043] The extraction rate of polysaccharide was determined to be 45%, the recovery rate of polysaccharide separated and purified by AB-8 macroporous resin was 97%, the decolorization rate and protein removal rate were 97% and 98%, respectively, and the purity of the final purified polysaccharide was 98%.

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Abstract

The invention discloses a method for extracting polysaccharide of dendrobium officinale. The method specifically comprises the following steps of uniformly mixing dendrobium officinale powder and a deep eutectic solvent according to a material and liquid ratio of 1g to (30 to 50)mL, wherein the ratio of hydrogen bond receptor to hydrogen bond donor is 3 to (1 to 18)mol / mol; adding 4 to 8mg / mL of at least one of cellulase and pectinase; extracting for 90 to 120min at the temperature of 30 to 60 DEG C; separating and purifying the extracting solution, so as to obtain the polysaccharide of dendrobium officinale. Compared with the traditional enzyme type water extracting method, the method has the advantages that by using the enzyme-assisted deep eutectic solvent to extract, the volatility islow, the stability is high, and the reaction system is mild; the content of impurities in the extracting solution is reduced, the loss of the solvent is reduced, the purifying burden is decreased, theextracting rate of polysaccharide is increased by 10% or above, and the content of polysaccharide is increased by 10% or above.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a method for extracting dendrobium officinale polysaccharides. Background technique [0002] According to "Shen Nong's Materia Medica" and "The Pharmacopoeia of the People's Republic of China", it is recorded that Dendrobium officinale is a perennial epiphytic herb, which has the functions of prolonging life, nourishing the stomach and promoting body fluid, nourishing yin and clearing heat, and is used for dry mouth, polydipsia, retching, and post-illness. If it does not retreat, the bones will be steamed and labored and hot, the eyes will be dark and unclear, and the muscles and bones will be flaccid and soft. It is listed as an endangered species in the "Chinese Plant Red Book" and "China's Endangered and Rare Plants". In addition, the large market demand and unreasonable exploitation of wild Dendrobium candidum lead to the depletion of rare and harsh wild ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/00C08B37/0003
Inventor 娄文勇梁静曾英杰宗敏华王洪峰程建华杨继国
Owner SOUTH CHINA UNIV OF TECH
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