Mining and application of SSR markers closely linked to rice salt-tolerance QTL on chromosome 5

A number chromosome, chromosome technology, applied in the field of agricultural science, can solve problems such as difficulty, complex genetic basis, slow progress, etc., to achieve the effect of speeding up the process, clear site location, and convenient identification

Active Publication Date: 2021-08-03
中垦种业股份有限公司
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AI Technical Summary

Problems solved by technology

Since the salt tolerance of rice is a comprehensive expression of various salt-tolerant physiological and biochemical reactions, it is a quantitative trait controlled by multiple genes, and the genetic basis is complex. It is difficult to improve the salt tolerance of rice by traditional breeding methods, and the progress is slow.

Method used

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  • Mining and application of SSR markers closely linked to rice salt-tolerance QTL on chromosome 5
  • Mining and application of SSR markers closely linked to rice salt-tolerance QTL on chromosome 5
  • Mining and application of SSR markers closely linked to rice salt-tolerance QTL on chromosome 5

Examples

Experimental program
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Effect test

Embodiment 1

[0030] At the beginning of April 2015, 12 rice varieties with obvious differences in salt tolerance were selected for many years of field planting to carry out the identification of salt damage in rice ponds in the greenhouse of Dongxin Farm. The fresh weight of the plant and the yield per plant at the ripening stage were used to obtain the salt tolerance index of 12 rice varieties.

[0031] The methods for identifying salt damage in rice ponds are as follows: (1) Salt pond preparation: use stainless steel to build the salt pond. The salt pond is a long groove with a height of 35cm, a width of 35cm, and a length of 2.5m. The non-salinized soil was crushed, sieved, and then dried in the sun. An equivalent amount of 200 kg of non-salinized soil was added to each salt pool, and the salt damage treatment group and the control group were set up respectively. The salt damage treatment group used 80 kg of 0.5% NaCl salt solution. Soil soaking, the control group used the same amount o...

Embodiment 2

[0037] In the first season of 2015, the excellent salt-tolerant rice variety Haixiang 030 was selected as the female parent, and the salt-sensitive variety Lianjing No. 4 was selected as the male parent, and hybrid F1 was obtained. The F2 segregation population, the F2:3 family was obtained by selfing of the F2 single plant in the 2016 season.

[0038] In May 2017, the rice pond salt damage identification method was adopted for the F2:3 family. The detection index selected the plant fresh weight (plant fresh weight, PFW) at the peak tillering stage of rice. Ben's salt tolerance index.

[0039] The leaves of Haixiang 030×Lianjing 4 parent, F1 and F2:3 mapping populations were taken, and the DNA was extracted by SDS crude extraction method, and 368 pairs of SSR primers that were roughly evenly distributed on the 12 chromosomes of rice in the laboratory were used. Parents, F1 and F2:3 mapping populations were amplified by PCR (the PCR reaction system for PCR amplification was: t...

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Abstract

The invention discloses the excavation and application of SSR markers closely linked with rice salt tolerance QTL on chromosome 5. In the present invention, the excellent salt-tolerant rice variety Haixiang 030 is used as the female parent, and the salt-sensitive rice variety Lianjing No. 4 is used as the male parent, and the F2 population is constructed as the positioning population. By using the rice pond salt damage identification method, the detection index selects rice tillers The fresh weight of the plants at the peak stage was obtained, and the salt tolerance index of each family and parent was obtained. The salt tolerance index and SSR marker data were analyzed by linkage analysis, and two SSR markers closely linked to the rice salt tolerance QTL on chromosome 5 were obtained. The band pattern data marked by the two primers on chromosome 5 can predict the salt tolerance of the plant, speed up the screening efficiency of salt-tolerant rice, and accelerate the process of rice salt-tolerant breeding.

Description

technical field [0001] The invention relates to the excavation and application of SSR markers closely linked with rice salt tolerance QTL on chromosome 5 and belongs to the field of agricultural science and technology. Background technique [0002] Since entering the 21st century, the rapid development of science and technology, industry and agriculture has improved people's living standards, but it has also brought about disadvantages that cannot be ignored, such as climate change, environmental pollution, soil degradation, and soil erosion. Especially in our country, the continuous growth of population has directly caused the continuous reduction of the existing cultivated land area, and also aggravated the destruction of soil structure and land salinization. Soil salinization has become an increasingly serious problem in global agricultural production, which not only reduces the available arable land area year by year, but also seriously affects the growth of crops. Acco...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 徐杰何水华张贤张硕杨泽坤王玲
Owner 中垦种业股份有限公司
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