Animal marrow macrophage extraction device

Abstract

The invention discloses an animal marrow macrophage extraction device, which comprises a rear end sleeve, a scraper rod, a front end sleeve and a scraper, wherein the rear end sleeve is fixedly connected with an internal thread ring, the scraper rod is fixedly connected with an external thread ring, the internal thread ring is in screwed connection with the external thread ring, the rear end sleeve is fixedly connected to a connector tube, the scraper rod is of a hollow cylindrical tubular structure, the connector tube extends through the rear end sleeve and then extends into the scraper rod,the front end sleeve is in screwed connection to the scraper rod, the inner side of the front end sleeve is fixedly connected with a support seat, the support seat is sleeved and connected with a rotating shaft, the rotating shaft is fixedly connected with a rotating ring, the rotating ring is fixedly connected with a sealing plate, the front end sleeve is in screwed connection to the scraper, andthe scraper is internally provided with a plurality of sets of through holes. The animal marrow macrophage extraction device has a simple structure and is convenient to use. Pancreatic enzyme is injected into the connector tube, and with the stripping function of pancreatic enzyme, the scraper is promoted to scrape macrophages adhering to a wall while macrophage damage is reduced, and macrophagesare easily blown into single macrophage suspensions, which facilitates subsequent passage of cells.

Description

Technical field [0001] The invention relates to the technical field of biomedical equipment, in particular to an animal bone marrow macrophage extraction device. Background technique [0002] Myocardial ischemia-reperfusion injury is the most common pathological injury during cardiac surgery, and its harm includes short-term and long-term effects. The burst of oxygen free radicals and calcium overload are the main mechanisms of recent myocardial ischemia-reperfusion injury, and local and systemic inflammatory reactions participate in the entire process of injury, and are the main reasons for postoperative arrhythmia, heart failure and even death. In the long term, tissue repair and reconstruction caused by injury can eventually lead to myocardial fibrosis, which affects heart function and reduces the quality of life. [0003] At present, myocardial protection measures are often limited to interventions for inflammatory injury, and there is a lack of intervention for long-term fibr...

AI Technical Summary

Problems solved by technology

[0004] Macrophages grow on the wall. The existing extraction methods generally use a cell scraper to scrape off the adherent cells attached to the culture flask. However, the scraped cells are difficult to blow into a single-cell suspension, which is very unfavorable for the cells to be recovered later. pass on

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