Method and system for determining colorectal tumor cells on basis of KRAS and NDRG4 genes

A colorectal tumor and gene technology, applied in biochemical equipment and methods, microbial determination/examination, instruments, etc., can solve the problems of low patient compliance, poor specificity, and low sensitivity, and achieve accurate and specific diagnosis. Effect

Active Publication Date: 2018-08-28
SHANGHAI REALBIO TECH CO LTD
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, the methods used for colorectal cancer screening mainly include colonoscopy, fecal occult blood det

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and system for determining colorectal tumor cells on basis of KRAS and NDRG4 genes
  • Method and system for determining colorectal tumor cells on basis of KRAS and NDRG4 genes
  • Method and system for determining colorectal tumor cells on basis of KRAS and NDRG4 genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Select clinical samples with different course distributions of colonoscopy cases from Lanxi Red Cross Hospital, the Second Affiliated Hospital of Xi'an Jiaotong University, Xijing Hospital, and Tangdu Hospital from 2016 to 2017, including 77 cases of colorectal tumors and 204 cases of asymptomatic people stool samples. The above samples were subjected to gene capture and high-throughput sequencing, and their clinical and pathological information were collected at the same time. Finally, the genome sequencing results and clinical information were comprehensively analyzed to construct a colorectal tumor cell detection model.

[0045] (1) Gene capture and high-throughput sequencing

[0046] Fecal samples were subjected to gene capture and high-throughput sequencing in our laboratory (see figure 1 ). Extract the genomic DNA of the sample to be tested, and use the probe to capture the target genes KRAS and NDRG4; use the bisulfite conversion method to convert the NDRG4 gen...

Embodiment 2

[0075] Listed 6 cases of volunteers who were treated in Lanxi Red Cross Hospital, the Second Affiliated Hospital of Xi’an Jiaotong University, Xijing Hospital, and Tangdu Hospital, the process of applying the model to obtain the consistency judgment of the variable results measured by NGS technology.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method and system for determining colorectal tumor cells on the basis of KRAS and NDRG4 genes. The method includes: acquiring the KRAS gene and NDRG4 gene of a to-be-determined sample, and performing methylation on the NDRG4 gene; sequencing the KRAS gene and the NDRG4 gene after the methylation to obtain a KRAS gene sequencing result and a NDRG4 gene sequencing result; performing first comparison on the gene sequence of a first target position in the KRAS gene sequencing result and first reference sequence to calculate KRAS gene mutation rate; performing second comparison on the gene sequence of a second target position in the NDRG4 gene sequencing result and second reference sequence to calculate NDRG4 gene methylation linkage rate; determining whether the to-be-determined sample has colorectal tumor cells or not according to the KRAS gene mutation rate and the NDRG4 gene methylation linkage rate. By the method and system high in sensitivity, whether the sample has the colorectal tumor cells or not can be determined fast, simply and specifically.

Description

technical field [0001] The invention relates to the field of molecular biology technology. Specifically, the present invention relates to methods and systems for identifying colorectal tumor cells based on KRAS and NDRG4 genes. Background technique [0002] Colorectal cancer (CRC) is a common malignant tumor, including colon tumors and rectal tumors, also known as colorectal tumors, refers to malignant tumors that occur in the colon or rectum of the human lower gastrointestinal tract, and is one of the major cancers that threaten human health. First, its incidence rate ranks the third among male tumors and the second among female tumors in the world. In my country, with the development of society and economy, changes in residents' living habits and dietary structure, the incidence and mortality of colorectal tumors in my country continue to rise. According to the 2015 cancer statistics in China, the number of new cases and deaths of colorectal cancer in my country ranks fi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G06F19/22C12Q1/6869
CPCG16B30/00C12Q1/6869
Inventor 毛瑞芳陈璟王云霞王君秦楠
Owner SHANGHAI REALBIO TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap