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A kind of rapid propagation method of stem segment tissue of freshwater sand pear

A stem section and freshwater technology, applied in the field of rapid propagation of freshwater pear stem section tissue, can solve the problems of unstable reproduction coefficient, low induced germination rate, poor quality of sprouts, etc., achieve simple and convenient configuration method and operation, and save propagation materials , The effect of low browning rate

Active Publication Date: 2021-07-23
HUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only Song Guanhua et al. (2016) have studied the explant culture of freshwater pear and Zeng Lingda et al. (2017) have studied the explant culture technology of freshwater pear in the tissue culture of freshwater pear. There are technical problems such as high browning rate, unstable reproduction coefficient, low induced germination rate, poor quality of sprouts, difficult rooting, and cumbersome operation in the technology of rapid multiplication and rooting of test tube seedlings.

Method used

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  • A kind of rapid propagation method of stem segment tissue of freshwater sand pear
  • A kind of rapid propagation method of stem segment tissue of freshwater sand pear
  • A kind of rapid propagation method of stem segment tissue of freshwater sand pear

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1) Pretreatment of explants

[0040] Collect the newly germinated young shoots with a length of 5 cm on freshwater sand pear plants in the wild, remove the young leaves immediately, put them in a sealed bag, put them in an ice box, and take them back. Rinse the surface of the shoots with dirt, dust, and insect eggs with tap water After waiting for sundries, soak in saturated detergent solution for 5 minutes, then rinse the detergent with tap water and change the water 3 times, then rinse with running tap water for 5 hours, and finally seal it with a sealed bag and place it in a refrigerator at 8°C spare.

[0041] 2) Training process

[0042] Step 1, Adventitious Bud Induction

[0043] Select the newly germinated shoots of freshwater pears and cut off the leaves as rapid propagation materials, place them in 0.1% mercuric chloride solution for disinfection for 10 minutes, then rinse them with sterile water for 5 times, and dry them with sterile filter paper. Use an ino...

Embodiment 2

[0052] 1) Pretreatment of explants

[0053] Remove the young leaves from fresh shoots of 7cm freshwater sand pears, put them in a sealed bag, put them in an ice box, and bring them back. Rinse the surface of the shoots with dirt, dust, insect eggs and other sundries, then soak them in saturated washing powder solution 7 minutes, rinsed 4 times, rinsed with running tap water for 8 hours, sealed with a plastic bag, and placed in a 9°C refrigerator for later use.

[0054] 2) Training process

[0055] Step 1, Adventitious Bud Induction

[0056] Adventitious bud induction: select the newly germinated new shoots of freshwater pears and cut off the leaves as rapid propagation materials, sterilize in 0.2% mercuric chloride solution for 13 minutes, then rinse with sterile water for 6 times, and blot dry with sterile filter paper After watering, use an inoculation knife to cut a stem segment with 2 buds and a length of 1.0 cm or a stem segment with a total of 2 buds and a terminal bud...

Embodiment 3

[0065] 1) Pretreatment of explants

[0066] Remove the young leaves from fresh shoots of 10cm freshwater sand pears, put them in a sealed bag, put them in an ice box, and bring them back. Rinse the surface of the shoots with dust, sand, insect eggs and other sundries, and then place them in a saturated washing powder solution. Soak for 10 minutes, rinse 5 times, rinse with running tap water for 12 hours, seal with a plastic bag, and store in a 10°C refrigerator for later use.

[0067] 2) Training process

[0068] Step 1, Adventitious Bud Induction

[0069] Select the budding stem section of freshwater pear and cut it as explant, sterilize it in 0.3% mercuric chloride solution for 16 minutes, then rinse it with sterile water for 6 times, dry it with sterile filter paper, and cut it with an inoculation knife. The stem segment with 3 buds in lateral buds or the stem segment with 3 buds in total from lateral buds and terminal buds was inoculated on the induction medium, and cult...

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Abstract

The invention discloses a method for rapid propagation of freshwater pear stem tissue. The technical process steps are as follows: step 1, induction of adventitious buds; step 2, induction of clustered buds and multiplication culture; step 3, induction of rooting. The method for rapid propagation of stem segment tissue of freshwater sand pear has the advantages of few breeding and cultivation steps, high propagation coefficient, fast propagation speed, small amount of propagation material, low browning rate, and is beneficial to the propagation of freshwater sand pear and the preservation of germplasm resources. The advantages of simplicity, convenience and low cost.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for rapid propagation of freshwater sand pear stem segment tissue. Background technique [0002] Danshui sand pear, originating in Huiyang District, Huizhou City, Guangdong Province, is the hometown of General Ye Ting. It is a famous sand pear in Guangdong named after the place name. Its fruit is resistant to storage, the fruit color is bright and moist, and the flesh is crisp and juicy. It is deeply favored by people and once enjoyed a good reputation in South of the Five Ridges. After the reform and opening up, with the development of industry in Huiyang District and the changes in the agricultural production structure of Huiyang District, and the local vigorously promoted the planting of litchi, longan and other fruit trees in the early 1990s, the cultivation area of ​​Danshui sand pear gradually decreased, and the industry of Danshui sand pear gradually declined. . ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 曾令达宋冠华曾勇山廖建良黄瑞华李彩华曾惠雯
Owner HUIZHOU UNIV
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