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Application of three annular RNAs in systemic lupus erythematosus biomarker

A technology of biomarker and lupus erythematosus, which is applied in the determination/examination of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc. It can solve the problems of poor detection sensitivity, inability to effectively monitor the disease and evaluate the prognosis, and achieve easy detection , improve sensitivity and specificity, and quantify the effect of precision

Active Publication Date: 2018-08-24
南昌大学第一附属医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, all the existing specific laboratory detection indicators for SLE diagnosis have shortcomings such as poor detection sensitivity, inability to effectively monitor the condition and evaluate the prognosis.

Method used

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  • Application of three annular RNAs in systemic lupus erythematosus biomarker
  • Application of three annular RNAs in systemic lupus erythematosus biomarker
  • Application of three annular RNAs in systemic lupus erythematosus biomarker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Chip expression analysis of circ0082688, circ0082689 and circ0054189 in whole blood of patients with systemic lupus erythematosus and healthy controls

[0017] 1. Objects and Methods

[0018] 1. Object

[0019] Three patients with systemic lupus erythematosus admitted to the Department of Rheumatology and Immunology, The First Affiliated Hospital of Nanchang University, Jiangxi Province, were all female. All patients with SLE met the diagnostic criteria for SLE established by the American College of Rheumatology (ACR) in 1997, and did not have other rheumatic, metabolic and endocrine diseases. Three healthy controls were physical examinations in the health care department of the First Affiliated Hospital of Nanchang University during the same period. Inflammatory diseases, infectious diseases and autoimmune diseases were excluded, and the age and sex ratios were matched with the case study subjects.

[0020] 2. Method

[0021] (1) Sample collection: Collec...

Embodiment 2

[0027] Example 2: Real-time quantitative PCR detection of the expression of circ0082688, circ0082689, and circ0054189 in whole blood of patients with systemic lupus erythematosus

[0028] 1. Objects and Methods

[0029] 1. Object

[0030] (1) Systemic lupus erythematosus group: A total of 74 patients with systemic lupus erythematosus in the Rheumatology and Immunology Department of the First Affiliated Hospital of Nanchang University, Jiangxi Province were collected, including 7 males and 67 females, with an average age of (40.5±14.5) years. All patients with SLE met the diagnostic criteria for SLE established by the American College of Rheumatology (ACR) in 1997, and did not have other rheumatic, metabolic and endocrine diseases.

[0031] (2) Healthy control group: 43 healthy people who underwent physical examination in the First Affiliated Hospital of Nanchang University during the same period were collected, including 7 males and 36 females, with an average age of (43.9±13...

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PUM

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Abstract

The invention provides application of three annular RNAs in a systemic lupus erythematosus biomarker. The three annular RNAs are respectively circ0082688, circ0082689 and circ0054189, and base sequences are respectively SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. Compared with the whole blood of a healthy control, the three annular RNAs are significantly highly expressed in the plasma of a systemiclupus erythematosus patient, the whole blood circ0082688, circ0082689 and circ0054189 expression level of the systemic lupus erythematosus patient is higher than the healthy control. According to theapplication of three annular RNAs in the systemic lupus erythematosus biomarker, primers screening the three annular RNAs are prepared, a new idea is provided for studying the pathogenesis of the systemic lupus erythematosus, and a new target object is also provided for the early diagnosis of the three annular RNAs.

Description

technical field [0001] The present invention pertains to the use of natural ribonucleotides, and more particularly, relates to the use of three circular RNAs in systemic lupus erythematosus biomarkers. Background technique [0002] Systemic lupus erythematosus (SLE) is a systemic autoimmune disease of unknown etiology characterized by immune inflammation, which occurs in women of childbearing age. The presence of multiple autoantibodies represented by antinuclear antibodies in serum and multi-organ involvement are its two main clinical features. The clinical manifestations of the disease are complex, the course of the disease is prolonged and repeated, and the relapse and remission alternate, and the pathogenic mechanisms of the disease, such as immune dysfunction and key links in the pathogenesis of the disease, are still unclear. SLE patients are increasing all over the world. According to the preliminary statistics of epidemiology in my country, there are about 860,000 t...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/113C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 罗清李俊明黄自坤
Owner 南昌大学第一附属医院
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