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Deubiquitinating enzyme for stabilizing BMI1 in glioma

A glioma, ubiquitin technology, applied in the field of disease treatment

Inactive Publication Date: 2018-08-24
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in glioma, the role and mechanism of USP22, especially its effect on tumor stemness, remains largely unknown

Method used

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  • Deubiquitinating enzyme for stabilizing BMI1 in glioma
  • Deubiquitinating enzyme for stabilizing BMI1 in glioma
  • Deubiquitinating enzyme for stabilizing BMI1 in glioma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Application of USP22 in the treatment of glioma

[0023] 1. Clinical samples

[0024] Thirty clinical glioma samples were obtained from patients who underwent neurosurgery at Xiangya Hospital from 2013 to 2015. Five cases of normal brain tissue were obtained from partial lobotomy decompressive surgery in patients with cerebral edema after traumatic brain injury. All specimens were obtained with the consent of the patients and were approved by the Ethics Committee of Xiangya Hospital.

[0025] 2. Cell culture

[0026] The established glioblastoma cell lines including LN229, U251, U87MG, A172 and HA normal astrocytic cell lines were purchased from the Cell Bank of the Chinese Academy of Sciences. The authenticity of these tumor cell lines was verified by short tandem repeat analysis. All cell lines were cultured in DMEM medium (Gibco, USA), supplemented with 10% (v / v) fetal bovine serum (Gibco), 100u / ml penicillin (Gibco), 100U / ml streptomycin (Gibco), in C...

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Abstract

The invention belongs to the field of treatment of diseases and in particular relates to a deubiquitinating enzyme for stabilizing BMI1 in glioma. The deubiquitinating enzyme is ubiquitin-specific protease 22 (USP22) and the formation of a BMI1 level inhibition glioma transplanted tumor can be reduced through knocking down expression of the USP22.

Description

technical field [0001] The invention belongs to the field of disease treatment, and in particular relates to a deubiquitinating enzyme for stabilizing BMI1 in glioma. Background technique [0002] Glioma is the most common primary intracranial tumor. Over the years, median survival has barely improved despite conventional treatment strategies including surgery, radiotherapy, and chemotherapy. The presence of a small subset of cells in gliomas known as glioma stem cells has been widely recognized as a major cause of drug resistance in glioblastoma. Therefore, specific targeted therapy, especially targeted therapy aimed at eliminating glioma stem cells, will be of great significance for the progress of treatment and improvement of prognosis. [0003] The polycomb gene B cell-specific Moloney murine leukemia virus integration site 1 (BMI1) is a core member of the polycomb repressive complex 1 (PRC1), which is mainly involved in the regulation of cell development and different...

Claims

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Application Information

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IPC IPC(8): C12N9/48A61K45/00A61P35/00
CPCA61K45/00A61P35/00C12N9/485C12Y304/19012
Inventor 仇冠中金卫林
Owner SHANGHAI JIAO TONG UNIV
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