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Research method of S.obliquus culture in mixotrophic mode for wastewater flue gas treatment

A technology for flue gas treatment and wastewater treatment, which is applied in the fields of food industry wastewater treatment, microorganism-based methods, biological water/sewage treatment, etc. It can solve the limited problems and achieve the effect of promoting the growth of Scenedesmus, improving productivity and sewage treatment efficiency.

Inactive Publication Date: 2018-08-21
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most of the current research mainly uses CO 2 Combined with municipal wastewater for the production of biofuel feedstocks, very limited research on the application of food wastewater to industrial wastewater

Method used

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  • Research method of S.obliquus culture in mixotrophic mode for wastewater flue gas treatment
  • Research method of S.obliquus culture in mixotrophic mode for wastewater flue gas treatment
  • Research method of S.obliquus culture in mixotrophic mode for wastewater flue gas treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The typical composition of the flue gas in this embodiment is CO 2 : 14.1%; CO: 300ppm; NO X :207ppm; SO X :53ppm

[0051] The inventive method step is as follows:

[0052]1) Inoculate the commercially available Scenedesmus S.obliquus in a 2L cylindrical container, which contains 1.7L of BBM medium, and the inoculation volume is 10% (V 接种物 / V 培养基 ), and then cultured in a light incubator for 10 days; the culture conditions were 23°C, white fluorescent lighting 110μmol.m 2 .s -1 And pass through 0.5L.min -1 The absorbance of the Scenedesmus suspension in the basal medium was adjusted to 0.5.

[0053] The composition of BBM medium is shown in the table below:

[0054] name

Concentration / (mg.L -1 )

name

Concentration / (mg.L -1 )

NaNO 3

250

K H 2 PO 4

175

K 2 HPO 4

75

MgSO 4 ·7H 2 o

75

CaCl 2 2H 2 o

25

NaCl

25

EDTA

50

KOH

31

FeSO 4 ·7H 2 o...

Embodiment 2

[0067] The typical composition of the flue gas in this embodiment is CO 2 : 14.1%; CO: 300ppm; NO X :207ppm; SO X :53ppm

[0068] The inventive method step is as follows:

[0069] 1) Inoculate the commercially available Scenedesmus S.obliquus in a 2L cylindrical container, which contains 1.7L of BBM medium, and the inoculation volume is 10% (V 接种物 / V 培养基 ), and then cultured in a light incubator for 15 days; the culture conditions were temperature 27°C, white fluorescent lighting 135μmol.m 2 .s -1 And feed 1.0L.min -1 The absorbance of the Scenedesmus suspension in the basal medium was adjusted to 1.0.

[0070] BBM culture medium composition is the same as embodiment 1

[0071] 2) Wastewater pretreatment: Secondary municipal sewage (MWW) and food wastewater (FW) use 0.2 μm nylon microfiltration to remove microorganisms and suspended solid particles, and perform 100°C autoclaving treatment.

[0072] Secondary municipal wastewater (MWW) is collected from municipal waste...

Embodiment 3

[0083] The typical composition of the flue gas in this embodiment is CO 2 : 14.1%; CO: 300ppm; NO X :207ppm; SO X :53ppm

[0084] The inventive method step is as follows:

[0085] 1) Inoculate the commercially available Scenedesmus S.obliquus in a 2L cylindrical container, which contains 1.7L of BBM medium, and the inoculation volume is 10% (V 接种物 / V 培养基 ), and then cultured in a light incubator for 13 days; the culture conditions were 25°C, white fluorescent lighting 125μmol.m 2 .s -1 And pass through 0.75L.min -1 The absorbance of the Scenedesmus suspension in the basal medium was adjusted to 0.8.

[0086] BBM culture medium composition is the same as embodiment 1

[0087] 2) Wastewater pretreatment: Secondary municipal sewage (MWW) and food wastewater (FW) are treated with 0.2 μm nylon microfiltration to remove microorganisms and suspended solid particles, and then undergo autoclaving at 115°C.

[0088] Secondary municipal wastewater (MWW) is collected from municip...

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Abstract

The invention discloses a research method of S.obliquus culture in a mixotrophic mode for wastewater flue gas treatment. The research method mainly comprises the following steps: inoculating S.obliquus, namely inoculating S.obliquus into a container, adding a BBM medium of which the inoculation volume (V inoculum / V medium) is 10%, and then placing in a light incubator to be cultured for 10 to 15 days; performing wastewater pretreatment, namely carrying out microfiltration to remove microorganisms and suspended solid particles from wastewater and performing autoclaved sterilization at the temperature of 100 to 121 DEG C; carrying out a basic culture experiment and a mixotrophic-mode culture experiment. Wastewater flue gas is utilized and treated by carrying out mixotrophic-mode culture of the S.obliquus, without adding nutrients additionally and biomass with cost benefits can be produced, so that the research method of the S.obliquus culture in the mixotrophic mode for the wastewater flue gas treatment is a dual strategy for sustainable development.

Description

technical field [0001] The invention relates to the technical field of biological treatment of wastewater, in particular to a research method for the cultivation of a concurrent model Scenedesmus for wastewater flue gas treatment. Background technique [0002] In recent years, microalgae have received extensive attention and have been used in processes such as wastewater treatment, CO2 reduction, and biofuel production [1] . Combining microalgae with existing wastewater treatment can effectively reduce pollutants in wastewater and simultaneously carry out biomass production. [0003] Microalgae as CO 2 Sequestration of plants offers multiple advantages, with higher photosynthetic efficiency than terrestrial plants and their biomass yielding high-value products. [2] release of high concentrations of CO from different industrial sectors 2 The exhaust gas can be used as an economical CO 2 source for biomass production from microalgae [3] . [0004] Under mixed-trophic co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C02F3/32C02F9/14C12R1/89C02F103/32
CPCC02F1/001C02F1/50C02F3/322C02F2103/32C12N1/12
Inventor 宋春风邱依婷张尔雅孙亚伟谢美莲范智超
Owner TIANJIN UNIV
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