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Formulating method and accurate application of microbial fertilizer dynamic inoculant formula

A technology of microbial fertilizer and microbial flora, which is applied in the determination/inspection of microorganisms, the preparation of organic fertilizers, biochemical equipment and methods, etc., can solve the problems of untargeted and single function of biological fertilizers, and achieve the purpose of promoting proliferation and promoting Metabolic reaction, effect of improving stability

Pending Publication Date: 2018-08-21
银川忆尔昱生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems of single function and lack of pertinence of existing biological fertilizers, the present invention provides a method for formulating a dynamic formula of microbial fertilizers, which determines unique microbial formulas according to the distribution of soil microorganisms in different regions, and conducts natural plants. Appropriate microbial fertilizers are obtained through fermentation, and the formulation of microbial agents is formulated according to the nature of the soil, which is targeted and unique

Method used

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  • Formulating method and accurate application of microbial fertilizer dynamic inoculant formula
  • Formulating method and accurate application of microbial fertilizer dynamic inoculant formula
  • Formulating method and accurate application of microbial fertilizer dynamic inoculant formula

Examples

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Effect test

Embodiment 1

[0036] A customer in Ningbo, Zhejiang provided soil samples and needed to customize biofertilizers that met their soil needs. First, the soil microbial database in Ningbo was constructed, and the distribution ratio information of microbial composition in 300 soil samples in the area was obtained through high-throughput 16S rRNA gene sequencing (the process is as follows: figure 1 with 2 shown), then determine the dynamic bacterial agent formula of biological fertilizer according to the following method (as image 3 shown):

[0037] (1) Soil sample treatment: Microbead crushing method was used to extract and purify the microbial DNA in the sample, and 515F / 806RPCR primers were used to amplify the V4 hypervariable region of the 16S rRNA gene of microbial DNA; the 515F / 806R PCR primers were 515F: GTGCCAGCMGCCGCGGTAA, 806R: GGACTACHVGGGTWTCTAAT.

[0038] (2) Determination of the total amount of microorganisms in the soil: extract the total DNA of microorganisms in the soil, th...

Embodiment 2

[0046] Customers in the Zhongning area of ​​Ningxia provided soil samples and needed to customize biofertilizers that meet their soil needs. First, the soil microbial database in Zhongning area of ​​Ningxia was constructed, and the distribution ratio information of microbial composition in 200 soil samples in the area was obtained through high-throughput 16S rRNA gene sequencing.

[0047] Then determine the dynamic bacterial agent formula of biological fertilizer according to the following method:

[0048] (1) Soil sample treatment: Microbead crushing method was used to extract and purify the microbial DNA in the sample, and 515F / 806RPCR primers were used to amplify the V4 hypervariable region of the 16S rRNA gene of microbial DNA; the 515F / 806R PCR primers were 515F: GTGCCAGCMGCCGCGGTAA, 806R: GGACTACHVGGGTWTCTAAT.

[0049] (2) Determination of the total amount of microorganisms in the soil: extract the total DNA of microorganisms in the soil, then use 16S rRNA as the target...

Embodiment 3

[0055] A customer in a certain area of ​​Jiangsu provided soil samples for growing grapes, and customized organic fertilizer microecology that met their soil needs. 1. Establish a soil microbial database in this area, and obtain the distribution ratio information of microbial composition in 260 soil samples in this area through high-throughput 16S rRNA gene sequencing. 2. Determine the formula of personalized bacterial agent for microbial fertilizer according to the following method:

[0056] (1) Soil sample treatment: Microbead crushing method was used to extract and purify the microbial DNA in the sample, and 515F / 806RPCR primers were used to amplify the V4 hypervariable region of the 16S rRNA gene of microbial DNA; the 515F / 806R PCR primers were 515F: GTGCCAGCMGCCGCGGTAA, 806R: GGACTACHVGGGTWTCTAAT.

[0057] (2) Determination of the total amount of microorganisms in the soil: extract the total DNA of microorganisms in the soil, then use 16S rRNA as the target sequence, use...

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Abstract

The invention discloses a formulating method and accurate application of a microbial fertilizer dynamic inoculant formula, and belongs to the field of personalized customization of micro-ecological fertilizer. The active composite microbial inoculant formula is optimized and formulated by performing biological information analysis on the original microbial flora of a soil sample; natural plants are fermented by the inoculant formula, the plant components are continuously fermented and heated under the action of the active composite microbial inoculant subjected to personalized construction, the plant components are completely decomposed and composted while harmful pathogens are killed, and high-quality biological fertilizer is obtained; the microbial fertilizer dynamic inoculant formula solves the traditional single production process, further optimizes and virtuously circulates resources, is completely and accurately applied to the field of modern agriculture, fills the gap of the industry, greatly accords with the situation of China and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of personalized customization of micro-ecological organic fertilizers, and more specifically relates to a method for formulating dynamic formulas of microbial fertilizers. Background technique [0002] Ecological fertilizer is organic solid waste (including organic waste, straw, manure, livestock, poultry manure, cake, agricultural by-products and solid waste from food processing) made by microbial fermentation, deodorization and complete decomposition. fertilizer. Rich in a variety of functional microorganisms and rich trace elements, it can improve soil structure and play a role in nutrition, conditioning and health care for crop growth. [0003] However, most of the current ecological fertilizers are fermented with a single strain or a blind (for different individuals, the proportion of the bacterial population remains unchanged) compound strains for fermentation. The composition of ecological fertilizer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F15/00C05F17/00C12Q1/6851C12Q1/06
CPCC05F11/00C05F17/00C12Q1/6851C05F11/08C12Q2531/113Y02W30/40
Inventor 尹星胡建中
Owner 银川忆尔昱生物科技有限公司
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