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Preparation method of carrot culture medium and application of carrot culture medium to culture of mother strains of edible mushrooms

A technology of parent seed cultivation and carrot, applied in the directions of botanical equipment and methods, application, cultivation, etc., can solve the problems such as the inability to provide high-quality edible fungus strains, the quality of the strains varies, the production process is cumbersome, etc. Simple preparation method and rich nutrition effect

Inactive Publication Date: 2018-08-21
山东惠民齐发果蔬有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, potato agar medium is usually used for mother seed cultivation, and potato agar medium has complicated manufacturing process, many components for medium and needs to be boiled to obtain medium, and in the process of boiling, it is easy to Cause nutrient loss, resulting in different quality of the strains produced by the mother species, and cannot provide high-quality edible fungus strains with consistent quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A preparation method for carrot culture medium, the steps are as follows:

[0027] (1) Cleaning and segmentation: Rinse the carrots, remove the fibrous roots on the surface, drain the surface water, and then divide to obtain carrot sticks for later use;

[0028] (2) Sterilization: Place absorbent paper at the bottom of the glass test tube. The volume of the absorbent paper accounts for 1 / 4 of the total volume of the test tube. Then put the carrot sticks obtained in step (1) into the glass test tube. The volume of the carrot stick accounts for 1 / 4 of the total volume of the test tube. 2 / 4 of the total volume, sealed, sterilized under high temperature and high pressure, and ready for use; the sterilization pressure is 0.14Mpa, the sterilization temperature is 130°C, and the sterilization time is 40min;

[0029] (3) Carrot culture medium: take out the glass test tube containing carrots sterilized in step (2), and cool to 26° C. to obtain carrot culture medium.

[0030] Th...

Embodiment 2

[0035] A preparation method for carrot culture medium, the steps are as follows:

[0036] (1) Cleaning and segmentation: Rinse the carrots, remove the fibrous roots on the surface, drain the surface water, and then divide to obtain carrot pieces for later use;

[0037] (2) Sterilization: Place absorbent paper on the bottom of the glass petri dish, the volume of the absorbent paper accounts for 1 / 4 of the total volume of the petri dish, then put the carrot pieces obtained in step (1) into the petri dish, seal it, and carry out High temperature and high pressure sterilization, the sterilization pressure is 0.13Mpa, the sterilization temperature is 120°C, and the sterilization time is 50min;

[0038] (3) Carrot culture medium: take out the glass petri dish containing carrots sterilized in step (2), and cool to 25° C. to obtain carrot culture medium.

[0039] The application of the carrot culture medium obtained according to the preparation method of the above-mentioned carrot cu...

Embodiment 3

[0044] A preparation method for carrot culture medium, the steps are as follows:

[0045] (1) Cleaning and segmentation: Rinse the carrots, remove the fibrous roots on the surface, drain the surface water, and then divide to obtain carrot sticks for later use;

[0046] (2) Sterilization: Place absorbent paper at the bottom of the glass test tube. The volume of the absorbent paper accounts for 1 / 4 of the total volume of the test tube. Then put the carrot sticks obtained in step (1) into the glass test tube. The volume of the carrot stick accounts for 1 / 4 of the total volume of the test tube. 2 / 4 of the total volume, sealed, and subjected to high temperature and high pressure sterilization, the sterilization pressure is 0.15Mpa, the sterilization temperature is 140°C, and the sterilization time is 30 minutes;

[0047] (3) Carrot culture medium: take out the glass test tube containing carrots sterilized in step (2), and cool to 28° C. to obtain carrot culture medium.

[0048] Th...

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Abstract

The invention provides a preparation method of a carrot culture medium. The preparation method comprises the following steps: (1) carrot cleaning and segmenting; (2) sterilization, namely placing an absorbent paper at the bottom of a glass ware, then, placing the segmented carrot into the glass ware, carrying out sealing, and conducting high-temperature and high-pressure sterilization; and (3) carrot culture medium preparing, namely taking out the sterilized glass ware filled with the carrot, and then, carrying out cooling to the room temperature to obtain the carrot culture medium. The carrotculture medium is applied to the culture of mother strains of edible mushrooms, so that excellent mother strains of the edible mushrooms are obtained. The preparation method of the carrot culture medium has the advantages of simplicity, high practicability, suitability for popularization and capability of completely retaining nutrients in the carrot; the carrot culture medium prepared by using the preparation method of the carrot culture medium has the advantages of simple components, low price, availability and uniform quality; and the characteristics of the mother strains of the edible mushrooms, obtained by applying the carrot culture medium to the culture of the mother strains of various edible mushrooms, conform to the characteristics of the excellent mother strains of correspondingedible mushrooms.

Description

technical field [0001] The invention relates to the technical field of cultivating edible fungus parent species, in particular to a method for preparing a carrot culture medium and the application of the carrot culture medium in the cultivation of edible fungus parent species. Background technique [0002] Edible fungi are large fungi that can be eaten by humans. They are not only delicious but also nutritious, such as lowering blood cholesterol, providing essential amino acids, and treating high blood pressure. Therefore, with the improvement of people's health awareness, eating Bacteria has attracted much attention as a health food. The strains of edible fungi are divided into parent species, original species and cultivated species, wherein the parent species is a strain obtained by directly cultivating the fruiting bodies of edible fungi or the mycelium of edible fungi for pure breeding. [0003] In the prior art, potato agar medium is usually used for mother seed cultiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/20
CPCA01G18/20
Inventor 刘德育吴元元时冰
Owner 山东惠民齐发果蔬有限责任公司
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