Making method of paraffin section of Rhododendron simsii seeds

A paraffin section and production method technology, which is applied in the preparation, sampling, measuring device and other directions of test samples, can solve problems such as difficulty in obtaining seed tissue structure, seed shedding, etc., saving time and various materials, and high tableting efficiency. , clear dyeing effect

Inactive Publication Date: 2018-08-17
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the technical problem that a large number of seeds are easy to fall off and it is difficult to obtain a complete seed tissue structure in the process of ma

Method used

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  • Making method of paraffin section of Rhododendron simsii seeds
  • Making method of paraffin section of Rhododendron simsii seeds
  • Making method of paraffin section of Rhododendron simsii seeds

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Embodiment Construction

[0018] The present invention will be described in detail below in conjunction with the examples, and those without special instructions in the examples are conventional methods.

[0019] A kind of paraffin section preparation method of azalea seed of the present invention, comprises the following steps:

[0020] (1) Material collection and fixation: take pollinated Rhododendron ovaries and soak them in FAA fixative solution for fixation.

[0021] The hardness is fixed for 7 to 30 days until the internal tissue of the ovary is completely discolored; the FAA fixative is composed of 70% v / v ethanol, formaldehyde and glacial acetic acid by 70% v / v ethanol: formaldehyde: the volume ratio of glacial acetic acid is 18 : Mixed in a 1:1 ratio.

[0022] (2) Softening: Take out the fixed Rhododendron ovary, wash it 2 to 3 times with clear water, and cut it into lengths of 0.2 cm to 0.5 cm along the central axis length direction of the Rhododendron ovary, with a width of Cut into 0.2cm~...

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Abstract

The invention discloses a making method of a paraffin section of Rhododendron simsii seeds. The making method is mainly characterized by immobilizing ovaries of Rhododendron simsii flowers that are pollinated, softening, staining, dewatering, dipping in paraffin, embedding, sectioning, spreading, pasting, drying with a glass slide, removing paraffin, and packaging the section. The making method allows a paraffin section of Rhododendron simsii seeds having clear staining and complete tissue structure to be made; the defect is overcome that an existing paraffin sectioning method that performs staining only after paraffin dipping, embedding, sectioning, pasting, spreading and paraffin removal causes proneness to shedding of massive seeds during staining, which causes incomplete seed structurein sections.

Description

technical field [0001] The invention relates to the technical field of paraffin sections, in particular to a method for preparing paraffin sections of rhododendron seeds. Background technique [0002] Rhododendron is a famous flower in the world, and using its wild germplasm resources to carry out distant hybrid breeding can effectively improve the ornamental quality and resistance of existing Rhododendron varieties. However, hybridization disorder, especially post-fertilization disorder, is an important reason that limits the success of distant hybridization of rhododendron. Paraffin section technology can study the development process of hybrid embryos at the cellular level, thus providing a reliable basis for distant hybrid breeding. [0003] At present, the paraffin sections of rhododendron roots, stems, leaves, fruits, and seeds all adopt the following operating procedures: (1) sampling, (2) fixing, (3) softening, (4) dehydration, (5) transparency, (6) ) wax dipping a...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/28G01N1/286G01N1/30G01N2001/2873
Inventor 解玮佳宋杰李世峰彭绿春王继华张露蔡艳飞李树发
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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