Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing sodium succinate by taking glucose as raw material

A technology of sodium succinate and glucose, applied in the field of microbial fermentation, can solve the problems of environmental pollution and high production cost of sodium succinate production process, and achieve the effect of reducing production cost

Pending Publication Date: 2018-08-17
山东兰典生物科技股份有限公司
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Specifically, the technical problem to be solved by the present invention is to provide a method for producing sodium succinate with glucose as a raw material, so as to solve the problems of high production cost and serious environmental pollution in the existing sodium succinate production process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A kind of method using glucose as raw material to produce sodium succinate, the method may further comprise the steps:

[0035] (1) Configuration of culture medium

[0036] ① Configure the seed medium

[0037] In the primary seed tank (1m 3 ) and secondary seed tanks (20m 3 ), the volume is half of the volume of the tank, and the composition of the seed medium is calculated according to the mass concentration: glucose: 20g / L, peptone: 10g / L, KH 2 PO 4 : 1g / L, MgSO 4 ·7H 2 O: 0.5g / L, flow into NaOH or / and Na with a mass concentration of 20% 2 CO 3 Suspension, keep the pH value of the system stable at 7.0, distill water to the required volume, preferably yeast peptone;

[0038] ② Configure the fermentation medium

[0039] In the fermenter (150m 3 ), the fermentation medium is configured with glucose as the carbon source, and the volume is one-third of the tank volume. Yeast extract: 1.5g / L, MgSO 4 : 0.8g / L, (NH 4 ) 2 HPO 4 : 3.5g / L, C 5 h11 NO 2 HCl: 1.3g...

Embodiment 2

[0052] A kind of method using glucose as raw material to produce sodium succinate, the method may further comprise the steps:

[0053] (1) Configuration of culture medium

[0054] ① Configure the seed medium

[0055] In the primary seed tank (1m 3 ) and secondary seed tanks (20m 3 ), the volume is half of the volume of the tank, and the composition of the seed medium is calculated according to the mass concentration: glucose: 20g / L, peptone: 10g / L, KH 2 PO 4 : 1g / L, MgSO 4 ·7H 2 O: 0.5g / L, flow into NaOH or / and Na with a mass concentration of 20% 2 CO 3 Suspension, keep the pH value of the system stable at 6.8, distill water to the required volume, preferably yeast peptone;

[0056] ② Configure the fermentation medium

[0057] In the fermenter (150m 3 ), the fermentation medium is configured with glucose as the carbon source, and the volume is one-third of the tank volume. Yeast extract: 1.5g / L, MgSO 4 : 0.8g / L, (NH 4 ) 2 HPO 4 : 3.5g / L, C 5 h 11 NO 2 HCl: 1....

Embodiment 3

[0070] A kind of method using glucose as raw material to produce sodium succinate, the method may further comprise the steps:

[0071] (1) Configuration of culture medium

[0072] ① Configure the seed medium

[0073] In the primary seed tank (1m 3 ) and secondary seed tanks (20m 3 ), the volume is half of the volume of the tank, and the composition of the seed medium is calculated according to the mass concentration: glucose: 20g / L, peptone: 10g / L, KH 2 PO 4 : 1g / L, MgSO 4 ·7H 2 O: 0.5g / L, flow into NaOH or / and Na with a mass concentration of 20% 2 CO 3 Suspension, keep the pH value of the system stable at 7.2, distill water to the required volume, preferably yeast peptone;

[0074] ② Configure the fermentation medium

[0075] In the fermenter (150m 3 ), the fermentation medium is configured with glucose as the carbon source, and the volume is one-third of the tank volume. Yeast extract: 1.5g / L, MgSO 4 : 0.8g / L, (NH 4 ) 2 HPO 4 : 3.5g / L, C 5 h 11 NO 2 HCl: 1....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for producing sodium succinate by taking glucose as raw material, and relates to the technical field of microbial fermentation. In the method, recombinant Escherichiacoli is taken as a fermentation strain. The method comprises the following steps: performing aerobic culture on the fermentation strain in a primary seed tank; performing aerobic-anaerobic combinationculture on the fermentation strain in a secondary seed tank; performing anaerobic fermentation on the fermentation strain in a fermentation tank to produce sodium succinate fermentation broth; extracting the sodium succinate fermentation broth to produce a sodium succinate product, wherein a fermentation culture medium is prepared by taking the glucose as a carbon source in the fermentation tank;a NaOH or / and Na2CO3 suspension is added into the primary seed tank, the secondary seed tank and the fermentation tank during operation. Compared with the prior art, the method has the advantages that the production cost of the sodium succinate is lowered by 5304.8 yuan / ton, no waste is produced in the whole production process, and clean production is performed.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for producing sodium succinate using glucose as a raw material. Background technique [0002] The molecular formula of sodium succinate is: C 4 h 4 Na 2 o 4 , mainly used in the food industry, used as umami agent, feed additive, reagent for laboratory analysis, pharmaceutical industry intermediate, flavoring agent, sour agent, buffer agent, mainly used in the preparation of ham, sausage, aquatic products, seasoning liquid, etc. . [0003] At present, the production of sodium succinate mainly adopts the following process: succinic acid reacts with sodium hydroxide to form sodium succinate, which is filtered, concentrated at high temperature, cooled to crystallize, centrifuged, and dried to finally obtain sodium succinate. [0004] There are following problems and shortcoming in existing sodium succinate production technology: (1) production cost is too...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C07C51/42C07C51/43C07C55/10C12R1/19
CPCC07C51/42C07C51/43C12P7/46C07C55/10
Inventor 刘振龙张乐山高金龙
Owner 山东兰典生物科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com