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Immunofluorescence tomographic detection card for detecting canine C-reactive protein and preparation method

A reactive protein and immunofluorescence technology, which is applied in the field of immunofluorescence chromatography detection card, can solve the problems that cannot fully meet the detection requirements, affect the accuracy of quantification, poor repeatability and stability, etc., and achieve fast detection speed and maintain stability , release full effect

Pending Publication Date: 2018-08-14
广州敏捷生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses colloidal gold as the tracer, and the immune marker relies on electrostatic adsorption, which has defects such as low sensitivity, difficulty in quantification, poor repeatability and stability, and cannot fully meet the clinical detection needs.
[0005] Chinese patent ZL201710468621.6 discloses a canine C-reactive protein fluorescence detection test strip, which is composed of a sample absorption pad, a conjugate release pad, a reaction film, a water absorption pad, and a bottom plate in sequence. Canine C-reactive protein monoclonal antibody-fluorescent microsphere marker, the reaction membrane includes a detection area and a quality control area, the detection area is coated with chicken anti-canine C-reactive protein polyclonal antibody, and the quality control area is coated There are goat anti-mouse anti-antibodies; but there are following disadvantages in this method: the detection line coating antibody is a polyclonal antibody, which may affect the specificity of detection; A marker: mouse anti-canine C-reactive protein monoclonal antibody-fluorescent microsphere marker, when the C-reactive protein in the sample changes, the C-line fluorescence value will change accordingly, which may affect the stability of C value and T / C value , which seriously affects the accuracy of quantification

Method used

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  • Immunofluorescence tomographic detection card for detecting canine C-reactive protein and preparation method
  • Immunofluorescence tomographic detection card for detecting canine C-reactive protein and preparation method
  • Immunofluorescence tomographic detection card for detecting canine C-reactive protein and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0059] The present invention provides an immunofluorescence chromatography detection card for detecting canine C-reactive protein, see Figure 1 to Figure 2 , Including a housing 7, the housing 7 is provided with an immunofluorescence chromatography detection card for detecting canine C-reactive protein, and the immunofluorescence chromatography detection card for detecting canine C-reactive protein includes a card bottom plate 1. A sample pad 2, a binding pad 3, a nitrocellulose membrane 4, and a water-absorbing pad 5 are sequentially connected to the bottom plate 1. The binding pad 3 is provided with a fluorescent microsphere-labeled CRP monoclonal antibody and a fluorescent microsphere-labeled sheep Anti-chicken IgY; the nitrocellulose membrane 4 is provided with a quality control C line coated with chicken IgY and a detection T line coated with CRP monoclonal antibody. The casing 7 is provided with a sample loading hole 72 adapted to the sample pad 2, and an observation wind...

Embodiment 2

[0061] This application provides another immunofluorescence chromatography test card for detecting canine C-reactive protein, please refer to Figure 1 to Figure 3 , Including a box body 9, the body 9 is provided with a sample diluent bottle 6, a straw 8 and a test card, the test card is located in the housing 7, the immunofluorescence layer for detecting canine C-reactive protein The analytical test card includes a bottom plate 1, on which a sample pad 2, a binding pad 3, a nitrocellulose membrane 4, and a water-absorbing pad 5 are sequentially connected. The binding pad 3 is provided with a fluorescent microsphere-labeled CRP monoclonal antibody and fluorescent Goat-resistant chicken IgY labeled with microspheres. The nitrocellulose membrane 4 is provided with a quality control C line coated with chicken IgY and a detection T line coated with a CRP monoclonal antibody. The casing 7 is provided with a sample loading hole 72 adapted to the sample pad 2 and an observation window...

Embodiment 3

[0064] The preparation method of an immunofluorescence chromatography test card for detecting canine C-reactive protein provided in Example 1 or 2 is to assemble at a temperature (18~26) ℃ and a humidity ≤ 30%. 1 is connected with nitrocellulose membrane 4, absorbent pad 5, binding pad 3 and sample pad 2 in sequence; cut the pasted large plate into 4.0mm wide test strips, and put them into the plastic card housing, namely the CDV test card; Each test card is placed in an aluminum film bag, 1 package of desiccant is added, and heat sealed and sealed for use.

[0065] The specific preparation method is as follows:

[0066] 1.1 Preparation of bonding pad

[0067] 1.1.1 Labeling of CRP monoclonal antibody

[0068] 1) Select microspheres: the microspheres are polystyrene microspheres containing a fluorescent mismatch of rare earth europium; density: 1.05g / cm3; refractive index: 1.59@589nm, 25°C; diameter 300nm; functional group: carboxyl group ; Appearance: white.

[0069] 2) Dilution of ...

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Abstract

The invention discloses an immunofluorescence tomographic detection card for detecting canine C-reactive protein and a preparation method and aims at providing an immunofluorescence tomographic detection card with high sensitivity and reliable detection result for detecting the canine C-reactive protein. The invention is characterized in that the immunofluorescence tomographi detection card comprises a bottom plate, wherein a sample pad, a bonding pad, a nitrocellulose membrane and a water absorbing pad are connected on the bottom plate in sequence; the bonding pad is provided with CRP (C-Reactive Protein) monoclonal antibodies labeled by fluorescent microspheres and goat-anti-chicken IgY labeled by fluorescent microspheres; the nitrocellulose membrane is provided with a quality control Cline for coating chicken IgY and a detecting T line for coating the CRP monoclonal antibodies. The immunofluorescence tomographi detection card belongs to the field of detection of animal epidemic diseases.

Description

Technical field [0001] The invention discloses an immunofluorescence chromatography detection card, specifically, an immunofluorescence chromatography detection card for detecting canine C-reactive protein. The invention also discloses the preparation of the immunofluorescence chromatography detection card method. Background technique [0002] C-reactive protein (CRP) is a component of the body's non-immune system, and plays an important diagnostic warning role in the acute phase of many mammalian diseases. The synthesis of CRP in the liver is mainly regulated by inflammatory factors such as IL-1, IL-6 and TNF-α. CRP concentration is not affected by age, gender and weight. In human clinical medical diagnosis, CRP has been used as a diagnostic marker for inflammatory or non-inflammatory diseases for more than 30 years. In veterinary clinics, the detection of canine CRP is also widely used in clinical applications, including the diagnosis and differential diagnosis of acute infe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/533G01N33/558
CPCG01N33/533G01N33/558G01N33/577G01N33/6893G01N2333/4737
Inventor 汤永平梁展鹏
Owner 广州敏捷生物技术有限公司
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