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Initial culture medium for Pleione bulbocodioides and preparation method thereof

A culture medium and a technology for Orchid dulcis, applied in the directions of botanical equipment and methods, horticultural methods, horticultural tools/equipment, etc., can solve the problems of a small number of fruit pods, a low pollination rate, and incomplete development of seed embryos. , to achieve the effect of overcoming hypoplasia, realizing factory production and increasing growth rate

Inactive Publication Date: 2018-08-03
康美药业(昆明)种质资源有限公司
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Problems solved by technology

[0003] At present, most of the solitary orchids circulating in the market are wild, and a few are artificially cultivated; most of the solitary orchids are propagated by divisions. In the natural environment, the pollen pollination rate is low, and the number of pods grown is also small, and the seed embryos are well developed. Incomplete, can only be seeded in culture medium under sterile conditions for tissue culture

Method used

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  • Initial culture medium for Pleione bulbocodioides and preparation method thereof
  • Initial culture medium for Pleione bulbocodioides and preparation method thereof

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preparation example Construction

[0024] The preparation method of the first-generation medium of Suanlanlan comprises,

[0025] Solution pre-preparation steps: prepare macroelement solutions, trace element solutions, iron salt solutions, and organic component solutions respectively;

[0026] Mixing step: mix the macroelement solution, trace element solution, iron salt solution, organic component solution, sucrose and agar powder, constant volume, sterilize, cool, and solidify to obtain the first generation culture medium of Dusuanlan.

[0027] As a further embodiment, in the solution pre-allocation step, the preparation method of the macroelement solution is as follows: potassium nitrate, ammonium nitrate, calcium chloride dihydrate, magnesium sulfate heptahydrate, and potassium dihydrogen phosphate are dissolved respectively , and then potassium nitrate, ammonium nitrate, magnesium sulfate heptahydrate, and potassium dihydrogen phosphate are mixed and stored in a 10L container, and calcium chloride dihydrate...

Embodiment 1-3

[0033] Example 1-3 The first generation culture medium of Suanlan

[0034] The raw materials were weighed according to the ratio in Table 1, and the preparation was carried out according to the following steps. The difference was that the ratio of the added raw materials was different, and the first-generation culture medium product of Suanlanlan was prepared. See Table 1 for details:

[0035] Table 1: Raw material ratio table of embodiment 1-3

[0036]

[0037]

[0038] The preparation method of the above-mentioned Suanlan primary culture medium includes,

[0039] Solution pre-preparation step: prepare macroelement solution, trace element solution, iron salt solution, organic component solution respectively; specific as follows: the preparation method of described macroelement solution is as follows: take potassium nitrate, ammonium nitrate, dihydrate chlorine Dissolve calcium chloride, magnesium sulfate heptahydrate, and potassium dihydrogen phosphate separately, the...

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Abstract

The invention discloses an initial culture medium for Pleione bulbocodioides and a preparation method thereof. The initial culture medium for Pleione bulbocodioides is a 1 / 2 MS culture medium, whereincane sugar with a concentration of 2.0 g / L and agar powder with a concentration of 4.8 g / L are added into the1 / 2 MS culture medium. When used for initial tissue culture, the initial culture medium provided by the invention has the advantages that the initial culture medium overcomes the problems of incomplete development of seed embryos, low reproduction rates in natural environments and the likeof seeds; and the initial culture medium can effectively improve the breeding speed breeding quantity of the seedlings of Pleione bulbocodioides, controls germplasm stability from the source of female parents, guarantees the quality of the seedlings, realizes industrial production of high-quality seeds and seedlings of Pleione bulbocodioides, meets ornamental and medicinal demands of Pleione bulbocodioides, and reduces excess digging of wild Pleione bulbocodioides.

Description

technical field [0001] The invention relates to a technique for raising seedlings by tissue culture, in particular to a first-generation culture medium for orchid albicans and a preparation method thereof. Background technique [0002] Pleione bulbocodioides (Franch.) Rolfe, Orchidaceae only Pleione bulbocodioides, ground or epiphytic herb. It grows in forests at an altitude of 1100-3500 meters or on rocky ground or moss-covered rocks at the edge of forests, and is also common on grassy slopes with slightly shaded gravel. Mainly distributed in the southwest of Sichuan, China (Yuexi, Yanyuan, Shimian, Xichang, Muli), the west to the north of Guizhou (Kaili, Yinjiang, Leishan, Guiyang, Fanjingshan, Panxian, Anlong, Wangmo, Nayong), northwestern to southeastern Yunnan (Gongshan, Weixi, Lijiang, Dali, Yangbi, Yongning, Mengzi, Wenshan), southeastern Tibet (Chawarong), and northern Myanmar. The rhizome is used as medicine, which has the effects of clearing heat and detoxificati...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 许冬瑾严新
Owner 康美药业(昆明)种质资源有限公司
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