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Methods and systems for controlling dna, RNA and other biological molecules passing through nanopores

A nanopore and molecular technology, applied in biochemical equipment and methods, nanotechnology for sensing, separation methods, etc., can solve the problems of not providing DNA movement, slowing down DNA translocation, and insufficient

Active Publication Date: 2018-07-31
UNIVERSAL SEQUENCING TECH CORP
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  • Claims
  • Application Information

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Problems solved by technology

This is good for academic research, but not enough for large-scale cheap high-throughput DNA sequencing and other commercial applications
The electro-mechanical approach proposed by Peng et al. (2011) uses a piezoelectric layer in the fabrication of synthetic nanopores to control the nanopore size within the nanopore, thereby slowing down DNA translocation, however, it does not provide information on how to precisely control DNA movement mechanism

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  • Methods and systems for controlling dna, RNA and other biological molecules passing through nanopores
  • Methods and systems for controlling dna, RNA and other biological molecules passing through nanopores
  • Methods and systems for controlling dna, RNA and other biological molecules passing through nanopores

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Embodiment Construction

[0044] Part I: Mechanical control of passage of DNA and other biomolecules through nanopores

[0045] The present disclosure provides methods for precisely controlling the movement of molecules, such as DNA, through a nanopore by mechanical means, or more specifically, by nanometer (nm) or sub-nanometer (nm) precision piezoelectric actuators or combinations of actuators, such as Methods for the movement of DNA through nanopores. Combined with electricity, this moves DNA into and out of the nanopore continuously or stepwise at a rate sufficient or slow enough for reliable base sensing, depending on the base sensing method chosen. For example: an ion current blocking base sensing approach using protein nanopores requires a sensing time of at least 1 ms (millisecond) per base.

[0046] Fundamentals - Balance of Forces

[0047] Basic principles such as figure 1 As shown, it uses single-stranded DNA (ssDNA) as an example.

[0048] The nanopore (500) is part of the nanopore chip...

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Abstract

The present disclosure provides, in one aspect, a device and a method for unit sequencing and / or analysis of a molecular sequence comprising attaching the molecular sequence to a plate and controllingthe progression of the molecular sequence through a pore of a nanopore chip, wherein the separation distance between the nanopore chip and the scan plate is controlled by a precision mechanical drive, and the molecular sequence is sensed as it progresses through the nanopore.

Description

technical field [0001] The present disclosure generally relates to methods of controlling the movement of molecules, such as DNA (deoxyribonucleic acid), RNA (ribonucleic acid), and proteins, among other biomolecules, through nanopores so that they can be detected, characterized, and / or sequenced. Background technique [0002] Since 454Life Sciences introduced the first second-generation DNA sequencing system in 2005, DNA sequencing has become one of the hottest technologies in the rapidly growing market. The main feature of second-generation DNA sequencing is its massively parallel sequencing capability with ultra-high throughput and short read length. It is faster and cheaper than the first generation technique, the Sanger method. However, the short read lengths of second-generation DNA sequencing make de novo assembly, genome-wide phasing, and structural variation analysis very difficult, limiting its ability to resolve complex DNA regions with repetitive or heterozygous...

Claims

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Application Information

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IPC IPC(8): B01D15/38B82Y15/00C02F1/469C25B9/06G01N30/34C25B9/17
CPCB82Y15/00C02F1/469G01N33/48721B01D15/3885B01D15/3809B01D15/3823C12Q1/6869C12Q2565/518C12Q2565/631C25B9/17
Inventor 雷明罗伯特·罗尔达陈州涛尼伽·霍王勇
Owner UNIVERSAL SEQUENCING TECH CORP
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