Method for producing β-carotene by fermentation of B. trispora and β-carotene

Abstract

The invention relates to a method for producing β-carotene by B. trispora fermentation and β-carotene, belonging to the field of fermentation engineering. The above method comprises: respectively cultivating B. trispora positive bacteria and B. trispora negative bacteria, simultaneously inoculating, fermenting, and using a regulator to control the pH value of the fermentation system to 5.8-6.2 during the fermentation process, semi-continuous culture, starting at the beginning of fermentation At 60h, feed and feed. Regulator is containing PO 4 3+ And does not contain acidic substances of metal ions and salts. The fermentation medium adopted in the fermentation process contains starch phosphate ester substances. This method is simple and low cost, by using PO 4 3+ And acidic substances without metal ions and salts control the fermentation pH and provide PO 4 3+ , to avoid bringing in excess K + , together with the use of starch phosphates to increase the production of β-carotene. The resulting β-carotene has a relatively high yield and is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of fermentation engineering, and in particular to a method for producing β-carotene by B. trispora fermentation and β-carotene. Background technique [0002] Existing fermentation methods usually use KH 2 PO 4 or K 2 HPO 4 Contain PO 4 3+ salt to increase phosphorylation levels and provide K + , enhance the permeability of cell membrane, and benefit the entry of nutrients into cells. Use KH 2 PO 4 or K 2 HPO 4 Contain PO 4 3+ If the amount of salt is too small, the phosphorylation level will be insufficient, which will greatly reduce the metabolic flux of carotenoids, and if it is too much, it will bring a large amount of K + , so that the cell membrane permeability of the bacteria becomes too strong, and β-carotene is easily transported from the intracellular to the extracellular, so that the accumulation rate of the product is correspondingly slowed down, and the unit production efficiency is reduced. ...

AI Technical Summary

Problems solved by technology

Use KH 2 PO 4 or K 2 HPO 4 Contain PO 4 3+ If the amount of salt is too small, the phosphorylation level will be insufficient, which will greatly reduce the metabolic flux of carotenoids, and if it is too much, it will bring a large amount of K + , so that the cell membrane permeability of the bacteria becomes too strong, and the β-carotene is easily transported from the intracellular to the extracellular, so that the accumulation rate of the product is correspondingly slowed down, and the unit production efficiency is reduced.

[0003] At the same time, the existing B. trispora fermentation of β-carotene is mainly based on single-tank fermentation. In view of its limited natural output and long fermentation cycle, it is necessary to develop a semi-continuous or continuous culture process to further reduce production. cost