Preparation method of polypeptide
A technology of crude peptide and peptide resin, which is applied in the field of large-scale preparation of anti-tumor polypeptide ATAP-M8, can solve the problems of inability to realize large-scale synthesis, achieve the effect of avoiding oxidation and improving product purity
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Embodiment 1A
[0087] The preparation of embodiment 1ATAP-M8 38 peptides
[0088] 1.1 Solid-phase peptide synthesis
[0089] Clean the sand core of the reaction kettle, soak it with absolute ethanol overnight, then rinse it with clean water until it is clean, and finally wash it twice with absolute ethanol, and drain it for later use. The ambient temperature is controlled at 25±2°C, and the process of de-Fmoc and amino acid condensation requires high-purity nitrogen bubbling throughout.
[0090] Resin transformation: Weigh 2kg of Rink Amide AM resin (substitution degree 0.33mmol / g), add it to a 100L polypeptide synthesis reaction kettle, measure 25L DCM and add it to the reaction kettle, completely immerse the resin in the DCM solvent, and swell overnight. Removal of Fmoc protecting group: extract 18L of 20% (v / v) piperidine / DMF solution into a drained reactor, and stir for 30 minutes. Washing: Put 18L DMF into the drained reactor, stir and drain, repeat the operation 5 times.
[0091] Co...
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