Magnetic bead mixed liquid, nucleic acid preservation method, nucleic acid extraction reagent and reagent kit

Abstract

The invention discloses magnetic bead mixed liquid. The magnetic bead mixed liquid is prepared from magnetic beads, protease, a denaturing agent, glycerinum, a buffer agent and a nucleic acid settlingagent. The invention further disclose a nucleic acid preservation method, a nucleic acid extraction reagent and a reagent kit. The components of the magnetic bead mixed liquid are pre-mixed accordingto certain proportions, corresponding volume can be taken for one time when nucleic acid extraction is conducted, the needed components can be uniformed added then, the dosage is more accurate, and operation is more simple; and the packaging cost and transportation cost can be lowered by pre-mixing the components according to the proportions. The magnetic bead mixed liquid can enable the temperature range for preserving the magnetic beads to be wider. The magnetic bead mixed liquid is adopted to serve as a nucleic acid (or carrier particles containing nucleic acid) solvent, extraction of nucleic acid can be monitored when nucleic acid is extracted, the step that a sample is added into the nucleic acid extraction reagent by using a magnetic bead method is saved, and meanwhile, the homogeneity of the concentration of nucleic acid being added among multiple holes can be guaranteed. Nucleic acid can be stably preserved by adopting the magnetic bead mixed liquid.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a magnetic bead mixture, a nucleic acid preservation method, a nucleic acid extraction reagent and a kit. Background technique [0002] Magnetic bead method nucleic acid extraction reagent is a high-tech product that combines biological science and nanomaterial science. It mainly uses nanotechnology to improve and modify the surface of superparamagnetic nanoparticles to prepare superparamagnetic silicon oxide nanomagnetic particles. beads. The magnetic beads can specifically recognize and efficiently combine with nucleic acid molecules on the microscopic interface. Using the superparamagnetism of silicon oxide nanospheres, under the action of chaotropic salts and an external magnetic field, they can be removed from blood, animal tissues, food, DNA and RNA are isolated from samples such as pathogenic microorganisms, which can be applied in various fields such as clinical dis...

AI Technical Summary

Problems solved by technology

[0008] At present, the Chinese patent application CN102559654A discloses a DNA preservation solvent, the solvent components include glycerol, TE buffer, 1-carboxy-N,N,N-trimethylbetaine, this DNA preservation solution has more components , the preparation is complicated and the cost is high

Another Chinese patent application CN106434636A discloses a novel DNA stabilization solution and its preparation method. The solution formula mainly includes potassium phosphate, glucose, disodium edetate and water, which can be stored for more than one year without degradation by agarose gel electrophoresis detection. , but based on agarose detection, it is impossible to judge the fragmentation of DNA and this method needs to be stored at -20°C or -80°C, and cannot be stored at room temperature or refrigerated

Images