Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

RNA (ribonucleic acid) extract liquid, preparation method and extraction method of oil palm fruit pericarp RNA

An extraction method and extraction solution technology, applied in the direction of DNA preparation, recombinant DNA technology, etc., can solve the problems of serious RNA degradation, difficult research and analysis, DNA pollution, etc., and achieve the effect of simple preparation method, good quality RNA, and less degradation

Pending Publication Date: 2018-07-27
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even if the RNA can be extracted, it still contains a lot of impurities, making it difficult to carry out subsequent research and analysis
Although the CTAB method can be used to extract RNA from oil palm fruit, this method is very cumbersome, takes a long time, the extracted RNA is seriously degraded, and contains a lot of DNA contamination, which affects further research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • RNA (ribonucleic acid) extract liquid, preparation method and extraction method of oil palm fruit pericarp RNA
  • RNA (ribonucleic acid) extract liquid, preparation method and extraction method of oil palm fruit pericarp RNA
  • RNA (ribonucleic acid) extract liquid, preparation method and extraction method of oil palm fruit pericarp RNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of RNA extraction liquid, the RNA extraction liquid of every 100ml comprises:

[0034]

Embodiment 2

[0036] A kind of RNA extraction liquid, the RNA extraction liquid of every 100ml comprises:

[0037]

[0038]

Embodiment 3

[0040] A kind of RNA extraction liquid, the RNA extraction liquid of every 100ml comprises:

[0041]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of biology, and discloses RNA extract liquid, a preparation method and an extraction method of oil palm fruit pericarp RNA. The RNA extract liquid is prepared from common reagents, the preparation method is simple, and the quality is stable and controllable. The RNA of the oil palm fruit pericarp is extracted by adopting the RNA extract liquid disclosedby the invention, the obtained oil palm fruit pericarp RNA has good integrity, good quality, less degradation and less impurities, and the extract time is greatly shortened.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an RNA extraction solution, a preparation method and an oil palm fruit RNA extraction method. Background technique [0002] Oil palm (scientific name: Elaeis guineensis Jacq.) is a perennial monocot and is a tropical woody oil crop. The pulp and kernel of oil palm are rich in oil. Among various oil crops, it is known as the "world oil king". [0003] Because oil palm fruit contains a lot of pigments, oils, polysaccharides and other ingredients, it is difficult to extract RNA. RNA with high purity, good integrity, and no impurity contamination is the basis for molecular biology research such as cloning and cDNA library construction. Some commonly used RNA extraction kits, including Invitrogen Trizol and Tiagen's RNAplant, are not suitable for the extraction of RNA from oil palm fruit. During the extraction process, the RNA is severely degraded and the extraction efficiency is extre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 肖勇夏薇周丽霞杨耀东李静
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com