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Method for purifying choline glycerophosphate

A technology of glycerol phosphatidylcholine and glycerol phosphatidylcholine, which is applied in the field of purification of glycerol phosphatidylcholine, which can solve problems such as difficult to achieve high efficiency and environmental protection, difficult to completely remove impurities, and large amount of solvent consumption , to achieve the effect of avoiding working hours and solvents, shortening operation time and high product purity

Inactive Publication Date: 2018-07-27
ZHONGSHAN BAILING BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the efficiency of the solvent extraction process is low, and it is often difficult to completely remove impurities in one extraction, requiring repeated extractions
It takes a long time to work and consumes a large amount of solvent
It is difficult to be efficient and environmentally friendly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for purifying glycerophosphatidylcholine, comprising the following steps:

[0036] A. Dissolving the crude glycerol phosphorylcholine in absolute ethanol to obtain a solution of the reaction system; the mass ratio of the absolute ethanol to the crude glycerol phosphorylcholine is 6:1.

[0037] B, add calcium chloride to the reaction system dissolving liquid in step A, stir, and form the calcium salt complex precipitation of glycerol phosphatidylcholine; The molar ratio of described calcium chloride and glycerol phosphatidylcholine is 1 :1. The temperature condition of the precipitation reaction was room temperature, and the reaction time was 12 hours.

[0038] C. The reaction solution in which the precipitation reaction was stopped in step B was lowered to room temperature, and filtered to obtain a filter cake; the filter cake was washed with absolute ethanol.

[0039] D. Put the filter cake in step C into a vacuum drying oven to dry to obtain the calcium sal...

Embodiment 2

[0044] A method for purifying glycerophosphatidylcholine, comprising the following steps:

[0045] A. Dissolving the crude product of glycerol phosphorylcholine in anhydrous methanol to obtain a solution of the reaction system; the mass ratio of the crude product of anhydrous methanol to glycerol phosphorylcholine is 20:1.

[0046] B. Add calcium oxide to the solution of the reaction system in step A, stir, and form a calcium salt complex precipitation of glycerol phosphatidylcholine; the molar ratio of calcium oxide to glycerol phosphatidylcholine is 1.5:1 . The temperature condition of the precipitation reaction was 60° C., and the reaction time was 2 hours.

[0047] C. The reaction solution in which the precipitation reaction was stopped in step B was lowered to room temperature, and filtered to obtain a filter cake; the filter cake was washed with absolute ethanol.

[0048]D. Put the filter cake in step C into an oven to dry to obtain the calcium salt of glycerophosphati...

Embodiment 3

[0053] A method for purifying glycerophosphatidylcholine, comprising the following steps:

[0054] A. Dissolving the crude product of glycerol phosphorylcholine in absolute ethanol to obtain a solution of the reaction system; the mass ratio of the absolute product of absolute alcohol to the crude product of glycerol phosphorylcholine is 10:1.

[0055] B. Add calcium sulfate to the solution of the reaction system in step A, stir to form a calcium salt complex precipitation of glycerol phosphatidylcholine; the molar ratio of the calcium salt to glycerol phosphatidylcholine is 1.2:1 . The temperature condition of the precipitation reaction was 40° C., and the reaction time was 7 hours.

[0056] C. The reaction solution in which the precipitation reaction was stopped in step B was lowered to room temperature, and filtered to obtain a filter cake; the filter cake was washed with absolute ethanol.

[0057] D. Put the filter cake in step C into an oven to dry to obtain the calcium ...

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PUM

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Abstract

The invention discloses a method for purifying choline glycerophosphate. The method is characterized in that the method comprises the following steps: A. a choline glycerophosphate crude product is dissolved in a solvent, in order to obtain a reaction system dissolving liquid; B. calcium salt is added into reaction system dissolving liquid in the step A with stirring, in order to form a calcium salt compound precipitate of choline glycerophosphate; C. a precipitation reaction in the step B is stopped and a reaction solution is cooled to a room temperature, and filtering is carried out in orderto obtain a filter cake; the filter cake in the step C is placed in a baking oven for drying, in order to obtain calcium salt of choline glycerophosphate. Purified calcium salt can be used for removing calcium ions and corresponding complex anions by using an ion exchange resin exchange adsorption process, in order to obtain purified choline glycerophosphate without calcium ions. A nanofiltrationprocess can be used for removing calcium ions and complex anions, in order to obtain purified choline glycerophosphate. The method aims at overcoming deficiencies in the prior art, and the method forpurifying choline glycerophosphate with short time substantially reduces solvent consumption and power cost and reduces workload.

Description

technical field [0001] The invention relates to a method for purifying glycerol phosphatidylcholine. Background technique [0002] Glycerophosphatidylcholine, referred to as GPC, is a member of the phospholipid family. Plays an important role in the health of cell membranes. Glycerophosphatidylcholine is very abundant in breast milk. It repairs damaged brain function, improves brain function in healthy individuals and rejuvenates aging brains. As a health product, it can promote the biosynthesis of acetylcholine and phosphatidylcholine in the brain, improve people's memory and cognitive ability, and can also effectively treat senile dementia diseases such as Alzheimer's disease. anti-aging nutrients". [0003] Since the content of glycerophosphatidylcholine in food is relatively small, it is difficult to extract it directly from natural products. Generally, chemical synthesis is required to produce glycerol phosphatidylcholine. The chemical synthesis method uses raw ma...

Claims

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Application Information

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IPC IPC(8): C07F9/10
Inventor 欧松彭廷春钟可玲王明宗
Owner ZHONGSHAN BAILING BIOTECHNOLOGY CO LTD
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