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A marine degrading bacterium capable of degrading polycyclic aromatic hydrocarbons and its application

A bacterial strain, Halomonas technology, applied in the direction of bacteria, microbe-based methods, microbes, etc., can solve problems such as restricted development and limited resource reserves

Active Publication Date: 2021-07-13
ZHEJIANG WANLI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the resource reserves of existing polycyclic aromatic hydrocarbon degrading strains are still very limited, which seriously limits the further development of environmental pollutant PAHs bioremediation technology.

Method used

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  • A marine degrading bacterium capable of degrading polycyclic aromatic hydrocarbons and its application
  • A marine degrading bacterium capable of degrading polycyclic aromatic hydrocarbons and its application
  • A marine degrading bacterium capable of degrading polycyclic aromatic hydrocarbons and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Collection of samples:

[0018] The mud sample is a sediment mud column collected by the East Pacific Combination Area WBC1306 (Side W154.235, latitude N10.016) at the East Pacific Combination Area.

[0019] (2) PAHS dissolution:

[0020] The polycyclic aromatic hydrocarbon compound is added to the triangular bottle of high pressure sterilization, and the acetone solution is added to the 37 ° C shaker overnight to the propanone solution, and the bacterial medium I is added. Polycyclic aromatic hydrocarbon compound naphthalene, Philippine, pyrene, fluoranthene final concentration is 200 mg / l, 100 mg / l, 50 mg / L and 20 mg / L.

[0021] The bacterial medium I is: 0.10 g of citrate, NaCl 19.45g, MGCL 2 5.90g, NA 2 SO 4 3.24G, CACL 2 1.80G, KCL 0.55g, Nahco 3 0.16g, KBR 0.08G, SRCL 2 34.00mg, H 3 BO 3 22.00mg, sodium silicate 4.00mg, NAF 2.40mg, (NH 4 ) NO 3 1.60mg, NA 2 HPO 4 8.00 mg, 1 ml of trace elements, 1 ml of vitamin composite solution, deionized water 1L, pH 7....

Embodiment 2

[0030] (1) Preparation of PAHs Solid medium: sublimation method:

[0031] Due to the strong hydrophobicity of the PAHs compound, only the physical properties of the organic solvent, resulting in the polycyclic aromatic hydrocarbons to be added to the solid medium in a usual method. To this end, polycyclic aromatic hydrocarbon solid medium is obtained by ease of sublimation of polycyclic aromatic hydrocarbon compounds.

[0032] The specific method steps are as follows:

[0033] (1) First fabrication of two aluminum dishes, the diameter of the dish is the same as the diameter of the petri dish, so that it can be exactly the aluminum disc.

[0034] (2) Prepare a constant temperature heater;

[0035] Place the constant temperature heater in the fume hood and placed the aluminum dish to preheat it half an hour.

[0036] (3) Take a proper amount of the polycyclic aromatic hydrocarbon compound in the bottom of the aluminum disc, and placed the plate containing the medium I above the alum...

Embodiment 3

[0040] Preliminary determination of the ability to degrade PAHS using a PAHs Philippine solid medium

[0041] Solid medium I is prepared, and a layer of philipphi-plated compound is plated with a layer of phipples by sublimation method. The previously enriched obtained a strain was inoculated in the II liquid medium to be placed in a shaker (25 ° C) 48 h. Take high-pressure sterilization of 5 mm sheet of sheets of 5 mm in the bacterial liquid to be placed in a 25 ° C thermostatic incubator. Depending on whether the strain grows on the PAHS solid medium and the transparent circle around the colony, the ability of the strain degrades the ability of PAHs. If it is grown, it will illustrate that the transparent circle appears to PAHS, such as the transparent circle around the colony, indicating that the strain has a PAHs degradation capability. Conversely, if there is no transparent circle around the colony, the strain does not have multi-ring aromatic hydrocarbon degradation capabili...

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Abstract

The present invention relates to a Halomonas bacterial strain Halomonas sp.BN3-1, characterized in that: its nucleotide sequence is as shown in SEQ ID NO.1, compared with the prior art, the present invention has the following advantages: The Halomonas bacterium of the present invention degrades polycyclic aromatic hydrocarbons faster, and the Halomonas bacterium is cultivated in 100ml culture medium (the solubility of phenanthrene is 100mg / L) for one week, bacterial strains BN1‑1, BF1 The degradation rates of ‑1 and BN3‑1 to phenanthrene were 30%, 33%, and 74%, respectively, and the strain BN3‑1 had a higher degradation rate.

Description

Technical field [0001] The present invention belongs to microbial repair is subject to contaminated soil and water technology, and in particular, the present invention relates to a degradation of polycyclic aromatic hydrocarbons and their applications. Background technique [0002] Polycyclic Aromatic Hydrocarbons, PAHs is an organic pollutant that is generally present in an environment. Because of its toxicity, mutation, until mutagenesis, carcinogenicity and difficult degradation, the environment and public health have caused huge harm. With the harm of multi-ring aromatic hydrocarbons on many aspects of humans, it is now gradually understanding, and more and more researchers are now committed to studying how to more effective to remove PAHs in the environment. Polycyclic aromatic hydrocarbons in natural environments are degraded by adsorption, volatilization, photodegradation and chemical oxidation. The ocean is an important collection of PAHS, and the PAHs in the land and the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02A62D101/20C12R1/01
CPCA62D3/02A62D2101/20C12N1/205C12R2001/01
Inventor 杨季芳陈勇陈吉刚
Owner ZHEJIANG WANLI UNIV
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