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Fermentation culture medium for increasing ganoderic acid A in liquid fermented ganoderma mycelium and fermentation method

A technology of Ganoderma lucidum mycelium and fermentation medium, which is applied in the field of edible and medicinal fungus production technology, which can solve the problems of autolysis of mycelium, decrease in output, increase in cost, etc., and achieve the effect of realizing batch production and simple and controllable production process

Active Publication Date: 2018-07-06
上海禾向健康科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the production of Ganoderma lucidum mycelium by liquid submerged fermentation technology is too long, when the synthesis of Ganoderma acid A is not achieved, mycelium autolysis will occur, resulting in a decrease in production and a substantial increase in cost.

Method used

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  • Fermentation culture medium for increasing ganoderic acid A in liquid fermented ganoderma mycelium and fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1), select the ganoderma lucidum mycelium strain:

[0039] 2) Shaker seed: keep the temperature at 25°C, dilute the strain into 300ml liquid culture medium with about 1 square centimeter plate with culture medium, put it on the shaker, and stir for 3 days, control the stirring speed to 100 rpm / min; the liquid medium includes 2% corn flour, 1% soybean flour, 1% glucose, 1% yeast extract, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 50ppm vitamin B1, and the balance is water.

[0040] 3), one-level amplification: keep the temperature at 25°C, inhale the culture medium stirred in step 2) into a 300L seed tank with liquid culture medium under negative pressure, and cultivate for 2 days;

[0041] 4), two-stage amplification: keep the temperature at 25°C, suck the stirred liquid culture medium in step 3) into a 1.5-ton seed tank with liquid culture medium under negative pressure, and cultivate it for 2 days to obtain Ganoderma lucidum hyphae body seed fluid...

Embodiment 2

[0048] 1), select the ganoderma lucidum mycelium strain:

[0049] 2) Shaking table seed: keep the temperature at 26°C, dilute the strain into 150ml liquid medium with about 1 square centimeter plate with culture medium, put it on the shaker, and stir for 4 days, control the stirring speed to 120 rpm / min; the liquid medium includes 4% of corn flour, 2% of soybean flour, 1% to 3% of glucose, 1% of yeast extract, 0.5% of potassium dihydrogen phosphate, 0.1% of magnesium sulfate, and vitamin B1 in terms of mass percentage. 100ppm, the balance is water.

[0050] 3), one-level amplification: keep the temperature at 26°C, suck the culture medium stirred in step 2) into a 300L seed tank with liquid culture medium under negative pressure, and cultivate for 3 days;

[0051] 4), two-stage amplification: keep the temperature at 26°C, suck the stirred liquid culture medium in step 3) into a 1.5-ton seed tank with liquid culture medium under negative pressure, cultivate for 3 days, and ob...

Embodiment 3

[0058] 1), select the ganoderma lucidum mycelium strain:

[0059] 2) Shaker seed: keep the temperature at 26°C, dilute the strain into 200ml liquid medium with about 1 square centimeter plate with culture medium, put it on the shaker, and stir for 3 days, control the stirring speed to 110 rpm / min; the liquid medium includes 3% corn flour, 2% soybean flour, 2% glucose, 1% yeast extract, 0.3% potassium dihydrogen phosphate, 0.08% magnesium sulfate, 75ppm vitamin B1, and The amount is water.

[0060] 3), one-level amplification: keep the temperature at 25°C, inhale the culture medium stirred in step 2) into a 300L seed tank with liquid culture medium under negative pressure, and cultivate for 2 days;

[0061] 4), two-stage amplification: keep the temperature at 25°C, suck the stirred liquid culture medium in step 3) into a 1.5-ton seed tank with liquid culture medium under negative pressure, and cultivate it for 2 days to obtain Ganoderma lucidum hyphae body seed fluid;

[00...

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Abstract

The invention discloses a fermentation culture medium for increasing ganoderic acid A in a liquid fermented ganoderma mycelium and a fermentation method. The fermentation culture medium contains the following components in percentage by weight: 2%-5% of corn flour, 1%-2% of soybean meal, 1%-3% of glucose, 0.1%-0.5% of potassium dihydrogen phosphate, 0.05%-0.1% of magnesium sulfate, 50ppm-100ppm ofvitamin B1, 200umol / L-300umol / L of jasmonic acid methyl ester, and the balance of water. By fermenting the ganoderma mycelium in the fermentation culture medium, the fermentation time can be prolonged, and a fermented ganoderma mycelium product of high-content ganoderic acid A can be obtained.

Description

technical field [0001] The invention belongs to the technical field of edible and medicinal fungus production technology, and specifically relates to a fermentation medium and a fermentation method for increasing the ganoderma acid A in liquid fermented ganoderma mycelia, and increasing the content of ganoderma acid A in the ganoderma mycelium. Background technique [0002] Ganoderma lucidum has been hailed as the "auspicious grass" by Chinese medical scientists in the past dynasties. There have been a lot of studies on the pharmacological effects of Ganoderma lucidum. Chemotherapy effect, sedative and tranquilizing effect, cardiotonic and anti-myocardial ischemia effect, blood lipid regulation effect, hypoglycemic effect, anti-asthma effect, liver protection effect, anti-hypoxia and anti-aging effect, etc. With the in-depth study of Ganoderma lucidum by modern science, so far, a total of more than 350 chemical components have been isolated, mainly polysaccharides, terpenes,...

Claims

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Application Information

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IPC IPC(8): C12P33/00C12N1/14C12R1/645
CPCC12N1/14C12P33/00
Inventor 孙怡凯
Owner 上海禾向健康科技发展有限公司
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