Compound enzyme protein peptide extraction method used for sesame

Abstract

The invention discloses a compound enzyme protein peptide extraction method used for sesame, comprising the following steps: (1) adding equal mass of compound enzyme to sesame dregs, and performing enzymolysis in an environment with temperature of 37 DEG C and pH value of 8.3, and carrying out enzymolysis for 2h, to obtain an enzymatic hydrolysate; (2) centrifuging the enzymatic hydrolysate for 15min at 4000rpm to obtain a sesame polypeptide mixed solution, and concentrating, freezing and reserving for later use. By adopting the manner, according to the compound enzyme protein peptide extraction method used for sesame, by adopting trypsin and acid protease with composition proportion of 3:1 as the compound enzyme, and performing enzymolysis experiment on the sesame dregs at the optimal temperature, pH and time, sesame protein peptide is extracted maximally, the method is novel, materials are saved, output rate is inreased, efficiency is increased, and the compound enzyme protein peptide extraction method used for sesame has wide market prospect in popularization.

Description

technical field [0001] The invention relates to the field of sesame protein peptide extraction, in particular to a compound enzyme protein peptide extraction method for sesame. Background technique [0002] Sesame is one of the four major edible oil crops in my country and one of the main oil crops in China. Sesame products have high application value. Its seeds contain as much as 61% oil. Since ancient times, there have been many famous special foods and delicacies made of sesame and sesame oil in our country, which have always been famous all over the world. [0003] Sesame, formerly known as flax, is said to have originated in Africa or India. According to legend, it was introduced to China by Zhang Qian in the Western Han Dynasty when he traveled to the Western Regions. But now through scientific research, sesame is native to the Yunnan-Guizhou Plateau in my country. In the Neolithic site of Qianshanyang in Huzhou City, Zhejiang Province and the prehistoric site of S...

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Problems solved by technology

Its reason is many-sided: 1. sesame is used as oilseed raw material, has restricted the development of sesame beverage industry to a certain extent; Soymilk Processing Technology", Xie Fanghua's "Study on Sesame and Soybean Mixed Milk Beverage", Chen Yan's "Development of Peanut, Soybean and Sesame Compound Milk Beverage", in these studies, sesame is only added to beverages as an auxiliary ingredient Medium; ③Although sesame has been developed into the above various forms of food, it cannot be ingested in large quantities, and it is inconvenient to easily ingest its active ingredients. In addition, because sesame contains a lot of oil, it is difficult to dissolve in water when it is used as a liquid food. Very bad dispersion stability, based on the above reasons, coupled with the nutritional and health properties of sesame itself and its unique flavor, research on the extraction and utilization of active ingredients in sesame, and the development of new sesame products not only have broad market prospects, but also It will bring consumers more healthy foods that consumers like, and can change the use of raw materials and crude products as finished products, and export refined products to obtain greater economic benefits

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