An arnebia root ointment determining method

Abstract

An arnebia root ointment determining method is disclosed. The method includes a step of weighing a medicine to be determined, adding a sodium hydroxide solution into the medicine, heating the solutionin a water bath under stirring, performing high-speed centrifugation after the solution turns blue to layer the solution, adding diluted hydrochloric acid into an obtained subnatant to adjust the subnatant until a red color occurs, performing extraction with trichloromethane, evaporating to dryness in a water bath, and adding 1 mL of methanol into residues to obtain a solution to be tested; a step of measuring arnebia root that is a contrast medicine material, adding sodium hydroxide into the contrast medicine material, performing extraction, and preparing a contrast medicine material solution through a same manner; and a step of measuring 10 [mu]L of the solution to be tested and 10 [mu]L of the contrast medicine material solution, spotting a silica gel G thin layer plate with the two solutions, performing development by adopting an upper-layer solution of petroleum ether-toluene-ethyl acetate-formic acid in a volume ratio of 3:8:2:1 as a developing agent, taking the silica gel G thin layer plate out, and airing the silica gel G thin layer plate, wherein in a chromatogram of the solution to be tested, spots having same colors are shown in positions corresponding to a chromatogramof the contrast medicine material. The method has advantages of good reproducibility, high specificity, an accurate result, and the like, meets principles of being accurate, simple, convenient, sensitive and rapid, and can effectively determine quality of the arnebia root ointment.

Description

technical field [0001] The invention relates to a method for determining medicine, in particular to a method for determining comfrey oil. Background technique [0002] Comfrey oil is a common external medicine, mainly used to treat chronic ulcers, diaper dermatitis, neurodermatitis, suppurative otitis media, burns and scalds, etc. According to clinical observations, the main component of comfrey - acetylshikonin It has astringent effect with shikonin and alkalized shikonin, and is effective for some skin injuries with excessive secretions, so it is applied to trauma and burn wounds; modern pharmacological studies have confirmed that comfrey has antibacterial and anti-inflammatory effects, and is effective for aureus Coccus, Escherichia coli, skin fungus, etc. all have inhibitory effects, and can accelerate epithelial growth and promote wound healing, so it is applied to infected wounds and neurodermatitis. [0003] The main component of comfrey oil is comfrey, and secondly,...

AI Technical Summary

Problems solved by technology

[0003] The main component of comfrey oil is comfrey, and secondly, it can also contain bletilla striata, angelica, burnet, phellodendron, etc. according to different formulas. At present, in the prior art, there is a method to measure comfrey oil by thin-layer chromatography. method, however, the applicant has found through research that the methods in the prior art or the steps of preparing the test solution are complicated, or the color spots cannot be clearly distinguished. Based on this situation, the applicant has developed through systematic research. A kind of determination method step is simple, easy to implement, and can effectively determine the determination method of comfrey oil

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