Measurement method for fructose and glucose contents in honey

A measurement method and technology for glucose, which are applied to measurement devices, material analysis and instruments by observing the impact on chemical indicators, can solve the problems of complicated steps, long measurement time, inconvenience for rapid detection and use, etc., and achieve good versatility. , Ease of operation, the effect of simple measurement steps

Inactive Publication Date: 2018-06-29
安徽泓顺源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Existing determination of fructose and glucose content in honey is mostly carried out by liquid chromatography. The determination method has complicated steps, long measurement time, and is not convenient for rapid detection and use. Therefore, we propose a method for the determination of fructose and glucose content in honey. Determination method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The invention discloses a method for determining the content of fructose and glucose in honey. The specific determination steps are as follows:

[0014] 1) Use a 0.1ml plastic straw to pipette evenly stirred liquid honey, drop 1 drop into a 5ml sample chromogenic tube, add distilled water to the 4ml mark of the sample chromogenic tube, cover it and shake it up and down 8 times to make Mix honey with water;

[0015] 2) Dip a piece of honey fructose and glucose test paper into the sample chromogenic tube solution for 8 seconds, remove excess solution along the inner wall of the sample chromogenic tube when taking it out, and compare it with the color scale card within 30 seconds.

[0016] In the step 1), if the honey has white crystals, it should be melted in a water bath at 50°C, stirred well, and then sampled for measurement.

[0017] After taking out the test paper in the step 2), compare it with the standard color guide within 30 seconds, and observe it in a place wi...

Embodiment 2

[0020] The invention discloses a method for determining the content of fructose and glucose in honey. The specific determination steps are as follows:

[0021] 1) Use a 0.3ml plastic straw to pipette evenly stirred liquid honey, drop 1 drop into a 5ml sample chromogenic tube, add distilled water to the 4ml mark of the sample chromogenic tube, cover it and shake it up and down 10 times to make Mix honey with water;

[0022] 2) Dip a piece of honey fructose and glucose test paper into the sample chromogenic tube solution for 10 seconds, remove excess solution along the inner wall of the sample chromogenic tube when taking it out, and compare it with the color scale card within 60 seconds.

[0023] In the step 1), if the honey has white crystals, it should be melted in a 60°C water bath, stirred well, and then sampled for measurement.

[0024] Take out the test paper in step 2) and compare it with the standard color guide within 60 seconds, preferably in a place with sufficient ...

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Abstract

The invention discloses a measurement method for fructose and glucose contents in honey. The measurement method comprises the following specific measurement steps: moving evenly stirred liquid honey by a plastic sucker of 0.1-0.3ml, dripping one drop of 5ml of sample into a color development pipe, adding distilled water to the 4ml-scale mark of the color development pipe, adding a cap, verticallyshaking for 8-10 times to evenly mix honey and water, dipping one piece of honey fructose and one piece of glucose test paper into sample color development pipe solution for 8-10 seconds, removing residual solution along the inner wall of the sample color development pipe when taking, and comparing with a color gradation card in 30-60 seconds. The measurement method has the technical scheme that the color of the test paper is observed to quickly distinguish the glucose and the fructose in the test sample honey so as to perform a purpose of quick measurement. The measurement method has the advantages of simple measurement step, easiness in operation and good universality.

Description

technical field [0001] The invention relates to the technical field of honey detection, in particular to a method for measuring fructose and glucose contents in honey. Background technique [0002] The sugar in honey is mainly monosaccharides, namely glucose and fructose, which usually account for more than 65% of the total composition of honey. Glucose and fructose in honey are called reducing sugars because they are reducing, and they come from nectar The main source of glucose and fructose is the sucrose in nectar produced by the invertase secreted by bees, which endow honey with sweetness, hygroscopicity and energy value and tangible characteristics. In general, the glucose and fructose content in honey is about the same. Most of the less sugar in honey does not exist in nectar, they may be formed by the action of various enzymes and acids during the brewing process of honey, so the fructose in honey is a special kind of fructose . [0003] Existing determination of f...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/77
CPCG01N21/77G01N21/78G01N2021/7759
Inventor 汪昌宏
Owner 安徽泓顺源生物科技有限公司
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