Reagent for detecting viruses in tilapia mossambica lake, detection method and application

Abstract

The application discloses a reagent for detecting viruses in a tilapia mossambica lake, a detection method and application. The reagent comprises a specific primer pair and a probe, wherein an upstream primer of the specific primer pair is a sequence shown in Seq ID No. 1, a downstream primer of the specific primer pair is a sequence shown in Seq ID No. 2, a probe is a sequence shown in Seq ID No.3 or a reverse complementary sequence shown in Seq ID No. 3, wherein the 5' end of the sequence of the probe has fluorescent groups, and the 3' end of the sequence of the probe has fluorescent quenching groups. The application provides a new detection reagent with good specificity, high sensitivity and simple-convenient-safe use for the viruses in the tilapia mossambica lake, and the new detection reagent is especially applicable to inspection and quarantine of the viruses in the tilapia mossambica lake and fast detection of the viruses in the tilapia mossambica lake in production practice, and has an important significance for fast quarantine and control of the viruses in the tilapia mossambica lake and maximum guarantee of production and trade safety.

Description

technical field [0001] The application relates to the field of detection of tilapia lake virus, in particular to a reagent, detection method and application for detection of tilapia lake virus. Background technique [0002] Tilapia Lake Virus (TiLV for short) is a newly discovered virus in recent years, and its taxonomic status has not yet been confirmed. According to its biological characteristics, TiLV is currently classified as a new virus in the family Orthomyxoviridae. Virus particles are enveloped icosahedral structures with a size of 55-75 nm. TiLV is a segmented, single-stranded, negative-sense RNA virus consisting of 10 gene segments. Each fragment has an open reading frame (ORF) encoding 10 proteins. [0003] Tilapia lake virus is the pathogen that causes syncytial hepatitis of tilapia (SHT) in tilapia. Infection with TiLV can lead to a death rate of 70%-90% in tilapia. Tilapia is the second most important farming in the world. Fish is also an important farming ...

AI Technical Summary

Problems solved by technology

The first three detection methods are complicated to operate and take a long time. They are generally used in laboratory diagnosis, and their detection sensitivity is low. They can only be used for detection of diseased tilapia or tilapia that is about to become diseased.

Although the sensitivity of SybrGreenqRT-PCR detection method is slightly higher than that of the first three methods, it also has the disadvantage of low specificity and is prone to false positives, which also limits the popularization and application of this method in production to a certain extent.

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