Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Medicine based on brain-gut axis anti-depression caused by chronic unpredictable stress and application thereof

An anti-depression, seed technology, applied in the direction of microbial-based methods, drug combinations, medical preparations containing active ingredients, etc., can solve the problems of affecting function, the real active ingredients of bacteria cannot be released, and Clostridium butyricum is not seen

Active Publication Date: 2018-06-22
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent CN105012349A discloses a probiotic preparation for preventing and treating vascular dementia and its preparation method, which relates to the new application of Clostridium butyricum for preventing and treating vascular dementia. The pathogenesis of disease and depression is completely different. Depression is mainly in the form of a decrease in 5-HT content; and Clostridium butyricum uses Clostridium butyricum cells, which are wrapped in cell walls and cell membranes, and the real active ingredients of bacteria Often can not be released, affecting the performance of the function
There are currently no reports on the use of Clostridium butyricum in the treatment of depression

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation method of described Clostridium butyricum preparation is as follows:

[0020] (1) Seed solution preparation: pick a single colony of Clostridium butyricum (Clostridium butyricum) WZMC1016, the preservation number is CGMCC No.9831, inoculate it in the sterilized MRS liquid medium, and culture it anaerobically at 37°C for 24 hours, namely Obtain Clostridium butyricum WZMC1016 seed solution;

[0021] (2) Fermentation: the seed liquid that step (1) obtains is inserted the inoculum amount of 1% weight ratio into sterilized peptone 4%, yeast extract 2.5%, glucose 10%, low Polyfructose 1%, ammonium sulfate 0.1%, potassium dihydrogen phosphate 0.2%, dipotassium hydrogen phosphate 0.2%, manganese sulfate 0.02%, magnesium sulfate 0.02% and calcium chloride 0.002%, and the rest is in the liquid medium of deionized water , static anaerobic fermentation at 37°C for 24 hours;

[0022] (3) Collect bacteria: Centrifuge the Clostridium butyricum WZMC1016 fermentation b...

Embodiment 3

[0028] 1. Experimental method

[0029] 1. Experimental animals: C57BL / B6 mice, male, weighing 25-30 g.

[0030] 2. Establishment of animal models of chronic unpredictable mild stress: chronic unpredictable mild stress, in order to prevent mice from developing tolerance to single or regular stimuli, this experiment uses a variety of mild stimuli alternately 10 different stresses were randomly accepted for 28 days, including heat stimulation (45°C, 5min), cold water stimulation (4°C, 5min), tail clamping (1cm from the tip of the tail, 1min), restraint (2h), plantar Electric shock (50mV, 30s / time, 5min), day and night reversal, 45° tilt (24h), wet bedding (24h), water fasting, suspension (15min). The mice were weighed every week to observe their weight gain; the mice with depression were screened by sugar water preference test, forced swimming test and tail suspension test.

[0031] 3. Brain Tissue Extraction

[0032] Mice in each group were anesthetized with chloral hydrate (10...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to application of clostridium butyricum, specifically relates to application of a clostridium butyricum preparation in medicine for curing depression, and belongs to the field ofbiological pharmacy. The invention discloses a composition of the clostridium butyricum preparation for curing depression. The research of animal experiments indicates that the clostridium butyricumcan improve depression actions, the content of depression model mouse brain tissue 5-HT is increased, and an anti-depression effect can be developed. The medicine has the advantages of good curative effect and no toxic or side effect, and is worthy of popularization.

Description

technical field [0001] The invention relates to the use of Clostridium butyricum, in particular to the application of Clostridium butyricum preparations in medicine for treating depression, and belongs to the field of biopharmaceuticals. Background technique [0002] Depression is a mental illness characterized by persistent and significant low mood. The clinical symptoms are complex, including low mood, loss of interest, and decreased speech. The degree can range from mild sadness to severe despair, and even suicide. , has a serious impact on people's work and life, and is the disease with the highest suicide rate in psychiatry. With the development of society, the pace of life is too fast, the competition is too fierce, and the mental pressure of human beings is too great. The environmental factors that induce depression are increasing day by day, which will cause nervousness or anxiety and depression, and have a wide range of effects on the normal physiological activities...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/742A61P25/24C12N1/20C12N1/06C12R1/145
CPCA61K35/742C12N1/06C12N1/20
Inventor 孙晶刘佳明
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com