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Living body lossless sampling method for procambarus clarkia and application

A non-destructive sampling technology for Crayfish, applied in the fields of application, biochemical equipment and methods, climate change adaptation, etc.

Active Publication Date: 2018-06-22
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] When sampling economic crustacean breeding parents for molecular detection, the method of amputating limbs and taking muscle is usually used. This method causes the sampling target to be disabled, the vitality is reduced, and some individuals die due to infection. Therefore, this method is not suitable for shrimp and crab molecules. linked marker assisted breeding

Method used

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Experimental program
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Effect test

Embodiment 1

[0030] 1. In October 2016, 2,000 ovum-bearing parents of Procambarus clarkii were collected from a farm in Yandu District, Jiangsu Province. The individuals were healthy, growing well, with complete appendages. Use a tightenable plastic collar with a numbered number to buckle at the base of the claws of the Procambarus clarkii to number each brooding shrimp;

[0031] 2. For each marked individual, use clean and disinfected tweezers to collect 5-10 fertilized eggs of different developmental stages (a total of 15-30 eggs) in the front, middle and back three parts of the egg-bearing shrimp abdomen, and combine them in Quick-frozen in liquid nitrogen in an Eppendorf tube and brought back to the laboratory for temporary storage in a -80°C refrigerator;

[0032] 3. Genomic DNA was extracted from the fertilized eggs of Procambarus clarkii 2000 at different development stages, and the extraction method was Soil Genomic DNA Kit from Beijing Kangwei Century Bioreagent Co., Ltd.

[0033...

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Abstract

The invention discloses a living body lossless sampling method for procambarus clarkia and an application. Fertilized ova of a procambarus clarkia oogenesis parent are sampled to obtain a sample. Through detection with a specific economic character linked molecular marker, the oogenesis parent containing the target character linked molecular marker is screened for isolated cultivation, and the first generation of a procambarus clarkia breeding group is obtained through the artificial propagation, and the screening of the next generation of the linked marker is continuously performed on the group, so the breeding group of which the gene frequency of the target character linked marker is up to 100% can be obtained. The method is capable of solving a problem that a living body is losslessly sampled in the procambarus clarkia molecular linked marker assistant breeding, and providing a scientific feasible route for the procambarus clarkia molecular marker assistant breeding. A new variety of the procambarus clarkia with the excellent characters can be expected to be bred, and the breeding economic benefit of the procambarus clarkia is improved.

Description

technical field [0001] The invention relates to molecular marker assisted breeding technology of economic crustaceans, in particular to a method and application of live non-destructive sampling of shrimp and crabs with ovipositing habit. Background technique [0002] The growth of economic crustaceans is carried out through molting. Procambarus clarkii molts (shells) more than 20 times in a lifetime. It is difficult to use physical surface marking methods similar to fish in crustaceans. Family selection is in most It is difficult or even impossible to carry out economic crustaceans. At present, the breeding of economic crustaceans mainly adopts the method of group selection breeding. Traditional selective breeding has advantages in growth traits, but the efficiency of selective breeding is very low for traits such as reproduction and disease resistance due to low heritability. For traits with low heritability such as reproduction and disease resistance, marker-assisted bree...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/59A01K61/17C12Q1/6888
CPCA01K61/17A01K61/59C12Q1/6888C12Q2600/156Y02A40/81
Inventor 沈怀舜黄鹏丹胡亚成
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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