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Mycobacterium tuberculosis drug allergy detecting color developing agent and mycobacterium tuberculosis drug allergy detecting method

A technology for detecting Mycobacterium tuberculosis and its detection method, which is applied in the field of Mycobacterium tuberculosis drug susceptibility detection chromogenic agent and its drug sensitivity detection, and can solve the problem of long detection period, unfavorable rapid drug sensitivity of Mycobacterium tuberculosis, tuberculosis mycobacterium The complex mechanism of bacillus drug resistance and other issues

Pending Publication Date: 2018-06-19
WUHAN POLYTECHNIC UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Tuberculosis is one of the major infectious diseases in the world. Due to reasons such as irregular drug use, the proportion of multidrug-resistant tuberculosis and extensively drug-resistant tuberculosis has risen rapidly. The drug resistance mechanism of Mycobacterium tuberculosis is complex. Molecular biological methods cannot fully meet clinical requirements. Therefore, bacteriological drug susceptibility testing and minimum inhibitory concentration (Minimum inhibitory concentration, MIC) determination have become important means for anti-tuberculosis new drug screening and individualized precision treatment. In the process of drug susceptibility testing, it is necessary to use culture medium to cultivate Mycobacterium tuberculosis, but the drug susceptibility testing of Mycobacterium tuberculosis based on the existing culture medium generally takes at least 1 week, and the detection period is long, which is not conducive to the development of Mycobacterium tuberculosis. Rapid drug susceptibility testing

Method used

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  • Mycobacterium tuberculosis drug allergy detecting color developing agent and mycobacterium tuberculosis drug allergy detecting method
  • Mycobacterium tuberculosis drug allergy detecting color developing agent and mycobacterium tuberculosis drug allergy detecting method
  • Mycobacterium tuberculosis drug allergy detecting color developing agent and mycobacterium tuberculosis drug allergy detecting method

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Embodiment 1

[0046] 40 clinical isolates of Mycobacterium tuberculosis (including 10 strains each of sensitive strains, drug-resistant strains, multidrug-resistant strains and extensively drug-resistant strains) with clear drug susceptibility results (cultivated by solid culture method) were used as test strains. The XTT / mPMS Mycobacterium tuberculosis drug susceptibility detection chromogenic agent provided by the invention is a chromogenic agent, with anti-tuberculosis drugs rifampicin (RFP) and isoniazid (INH) as test drugs, to the Mycobacterium tuberculosis bacterial strain Evaluate the resistance to RFP, INH and RFP and INH at the same time. The specific operation methods are as follows:

[0047] (1) Preparation of Mycobacterium tuberculosis bacteria liquid:

[0048] Using 7H9 liquid medium containing 0.05% Tween 80 and 10% OADC as the medium, after activating the Mycobacterium tuberculosis strain, pick a single colony and transfer it to 7H9 liquid medium for cultivation to Mycobacter...

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Abstract

The invention discloses a mycobacterium tuberculosis drug allergy detecting color developing agent and a mycobacterium tuberculosis drug allergy detecting method. The color developing agent contains 3,3'-[1-(benzene aminoacyl)-3,4-tetrazole]-bis(4-methoxyl-6-nitro)sodium benzenesulfonate and 1-methoxyl-5-methylphenazinium methyl sulfate. According to the mycobacterium tuberculosis drug allergy detecting color developing agent, soluble formazan is generated through reaction of 3,3'-[1-(benzene aminoacyl)-3,4-tetrazole]-bis(4-methoxyl-6-nitro)sodium benzenesulfonate and redox metabolite of thallus, and rapidly penetrates through a cytomembrane through an intermediate electron acceptor, namely 1-methoxyl-5-methylphenazinium methyl sulfate, so that the rapid drug allergy detection of mycobacterium tuberculosis can be realized.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a color developing agent for drug sensitivity detection of Mycobacterium tuberculosis and a drug sensitivity detection method thereof. Background technique [0002] Tuberculosis is one of the major infectious diseases in the world. Due to reasons such as irregular drug use, the proportion of multidrug-resistant tuberculosis and extensively drug-resistant tuberculosis has risen rapidly. The drug resistance mechanism of Mycobacterium tuberculosis is complex. Molecular biological methods cannot fully meet clinical requirements. Therefore, bacteriological drug susceptibility testing and minimum inhibitory concentration (Minimum inhibitory concentration, MIC) determination have become important means for anti-tuberculosis new drug screening and individualized precision treatment. In the process of drug susceptibility testing, it is necessary to use culture medium to cultivate M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/32
CPCC12Q1/04
Inventor 余晓丽孙战强张舒林
Owner WUHAN POLYTECHNIC UNIVERSITY
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