Traditional Chinese medicine composition for preventing and treating chicken coccidiosis as well as preparation method, use method and application of traditional Chinese medicine composition
A technology for chicken coccidiosis and a composition is applied in the field of traditional Chinese medicine compositions for preventing and treating chicken coccidiosis and the field of preparation thereof, which can solve the problems of hidden dangers of chicken products, affecting the safety of chicken products, flooding of drug use, etc. Soothing, good curative effect
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Embodiment 1
[0018] Prepare the traditional Chinese medicine composition that is used for preventing and treating chicken coccidiosis, according to the formula in Table 1, adopt the following method to prepare: according to each formula in Table 1, weigh Verbena, Phellodendron Cortex, Gardenia, Rhizoma Imperatae and Atractylodes Rhizome respectively, and then After mixing the traditional Chinese medicines, pulverize them, pass through a 65-mesh sieve and take the sieved matter.
[0019] Table 1 is used for preventing and treating the Chinese medicine composition formula of chicken coccidiosis
[0020]
[0021]
Embodiment 2
[0023] Influence of each Chinese medicine composition prepared in embodiment 1 on sporulation rate of Eimeria tenella in vitro
[0024] Weigh each 10g of the traditional Chinese medicine composition prepared by the 5 formulas, add 300mL of purified and sterilized water, soak for 30min, boil on direct fire and maintain a simmer for 30min, centrifuge to remove the medicinal residues, heat and concentrate each medicinal solution to 100mL (concentration is 0.1 g / ml) to make 5 groups of oocyst treatment solutions.
[0025] Take 21 sterilized test tubes, divide them into 7 groups, each group has 3 repetitions, numbered A1-H1, A2-H2, G1-G3, add 1mL containing 2.51×10 5 A suspension of unsporulated Eimeria tenella oocysts; add 1 mL of oocyst treatment solution prepared according to formula 1 and 3 mL of purified water to A1-A3, add 1 mL of oocyst treatment solution prepared according to formula 2 to B1-B3 and 3 mL of purified water, add 1 mL of oocyst treatment solution prepared acco...
Embodiment 3
[0030] Effect of traditional Chinese medicine composition prepared by formula 4 on the sporulation rate of Eimeria tenella in chickens
[0031] Check the feces of the chicks before infection, select 60 5-day-old chicks with similar body weight (the difference in body weight does not exceed ± 5g) and confirm that there is no coccidia infection by observation, and feed them adaptively under the same conditions for 6 days (that is, 11 days). age), and then 60 11-day-old chicks were randomly divided into 6 groups, 10 in each group.
[0032] At the age of 12 days, the 1st group to the 3rd group were the test groups, and the feed added with the Chinese medicine composition made by formula 4 in Example 1 was fed until the end of the test, wherein, in the 1st group, 10g per kilogram of feed was added. For the Chinese medicine composition, 15 g of the Chinese medicine composition was added to every kilogram of feed in the second group, and 20 g of the Chinese medicine composition was a...
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