Application of allograft acellular dermal matrix in oral diseases
A dermal matrix and decellularized technology, which is applied in the field of tissue engineering of biomedical materials, can solve the problems of affecting wound healing and functional recovery, affecting function and shape, and large surgical trauma, so as to reduce cytotoxicity and rejection, without Immune rejection, no contour effect
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Embodiment 1
[0050] Example 1 Preparation method of acellular allodermal matrix
[0051] Put allogeneic leather raw materials into 0.3g / L phospholipase and 0.1g / L trypsin solution, the pH value of the enzyme solution is 7.0, the temperature is 37°C, shake at 100rpm for 2 hours, replace the enzyme solution once, repeat this process once, The semi-finished product A is obtained; the phospholipase is a mixture of phospholipase A1: phospholipase A2: phospholipase C: phospholipase D according to the mass ratio of 1:1:1:1.
[0052] The semi-finished product A was taken out, put into a container filled with physiological saline solution, soaked in physiological saline, oscillated 3 times, each time for 2 hours, the oscillation speed was 80 rpm, and the temperature was 2°C to obtain the semi-finished product B.
[0053] Put the semi-finished product B into the solution containing 0.2g / LTritonX-100, 50KHz, 220W, ultrasonic for 5 minutes, soak for 3 hours, repeat this process 3 times, and get the se...
Embodiment 2
[0059] Example 2 Preparation method of acellular allogeneic dermal matrix
[0060] Put allogeneic leather raw materials into 0.2g / L phospholipase and 0.2g / L trypsin solution, the pH value of the enzyme solution is 7.0, the temperature is 37°C, shake at 100rpm for 2 hours, replace the enzyme solution once, repeat this process once, The semi-finished product A is obtained; the phospholipase is a mixture of phospholipase A1, phospholipase A2, phospholipase C and phospholipase D according to the mass ratio of 1:1:1:1.
[0061] The semi-finished product A was taken out, put into a container filled with physiological saline solution, soaked in physiological saline, oscillated 3 times, each time for 2 hours, the oscillation speed was 80 rpm, and the temperature was 2°C to obtain the semi-finished product B.
[0062] Put the semi-finished product B into a solution containing 0.2g / LSDS, 10mM EDTA, 50KHz, 220W, ultrasonic for 7 minutes, soak for 2 hours, repeat this process 3 times, and...
experiment example 1
[0070] Experimental example 1 Acellular allogeneic dermal matrix (oral patch) used in gingival defect surgery
[0071] Case 1
[0072] Li, male, 59 years old, had gingival defects in the left upper incisor and right upper incisor. The largest defect area was 1.0cm×0.8cm, and the smallest was 0.2cm×0.1cm. The gingival flap was performed under local anesthesia, and the surgical wound was completely hemostasis. Take the acellular allogeneic dermal matrix prepared in Example 1, which is slightly larger than the defect surface, soak it in normal saline and wash it, cut 2 small incisions in the center to facilitate drainage, attach the rough surface to the wound surface, and suture intermittently between the edge of the patch and the wound edge. Stay long. After the suture is completed, use the long thread left to wrap the iodoform oil gauze back and pressurize it, so that the patch is closely attached to the wound. On the 10th day after the operation, the iodoform bag was opened,...
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