Systems and methods for automated analysis of cellular phenotypic responses

Abstract

Disclosed are systems and methods for analyzing the phenotypic response of cells to treatment, wherein a model development module receives images of a plurality of reference cell vectors and treatment information associated with the plurality of reference cell vectors, identifying those in the images that will have been applied Those reference cell vectors treated are distinguished from the other reference cell vectors by the parameters of the cells, and the identified parameters are used to train the model. The high-content imaging system includes an image capture device, and the image acquisition module receives a plurality of images of the cellular carrier to be evaluated from the image capture device. The model application module applies the trained model to multiple images of the cell vectors being evaluated to predict the concentration of treatment applied to each cell vector being evaluated.

Description

Systems and methods for automated analysis of cellular phenotypic responses [0001] Related applications [0002] This application claims the benefit of U.S. Patent Application Serial No. 62 / 204,225, filed on August 12, 2015, which The contents are incorporated herein by reference in their entirety. technical field [0003] The present subject matter relates to high-content imaging systems and, more particularly, to systems that automate the analysis of the phenotypic response of cells system and method. Background technique [0004] Researchers can use high-content imaging systems (HCIS) to analyze biological cells for treatment (eg, exposure to specific phenotypic responses to certain substances and / or other environmental changes). These responses may be visually distinct and may Observable in images acquired with fluorescent labels and other techniques. However, this response can vary depending on the pathway involved in the response, cell tissue type, cell age, g...

AI Technical Summary

Problems solved by technology

The number of response types and the number of parameters that must be tuned in order to quantify each response can place a significant burden on researchers who must identify the phenotypic type of response that occurs and then manipulate the various parameters associated with that type. parameter to determine whether such a reaction did occur

[0006] Furthermore, in some cases it may not be practical or even possible to expose a homogenous population of target cells to the substance or environmental condition to be tested

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