Free fatty acid detection kit and preparation method thereof

Abstract

The invention discloses a free fatty acid detection kit and a preparation method thereof. The kit comprises reagents A and B, wherein the reagent A is prepared from the following components: sodium dihydrogen phosphate, a coenzyme A, triphosadenine, acyl coenzyme A synthetase, MgCl2, Trinder substrate and a proper amount of magnetized water, and the pH value is regulated to 7.2. The reagent B is prepared from the following components: sodium dihydrogen phosphate, 4-aminoantipyrine, acyl coenzyme A oxidase, peroxidase, flavin adenine dinucleotide, EDTA-2Na, N-sodium cocoanutylglutamate, sodiumlauryl ether sulphate, sodium N-acyl-L-serine, mannitol, sodium citrate and a proper amount of magnetized water, and the pH value is regulated to 7.2. The kit has the advantages of high and stable enzymatic activity and long preservation life; after the kit is preserved for about 15 days at 35-37 DEG C, the deviation of the detection result is only 1.99%; and the sensitivity is improved by 3.45%.

Description

technical field [0001] The invention belongs to the technical field of medical detection, in particular to a free fatty acid detection kit and a preparation method thereof. Background technique [0002] Free fatty acids, that is, non-esterified fatty acids, are intermediate products of fat metabolism in the human body, substrates of cell membrane lipid structures, and donors of intracellular signaling molecules such as prostaglandins. When glycogen, the energy source for muscle activity, is depleted, adipose tissue will decompose neutral fat into free fatty acids for energy use. Although free fatty acids only account for a small part of body fat, they meet a large part of energy demand, so they are important indicators for monitoring body fat metabolism and glucose metabolism. Free fatty acids can not only reflect the body's fat metabolism and blood lipid levels, evaluate blood sugar and assist in the diagnosis of diabetes, but also reflect a variety of other pathological a...

AI Technical Summary

Problems solved by technology

The report uses two non-ionic surfactants "alkyl glycoside APG0810 and lauryl polyoxyethylene ether" in combination with EDTA-2Na and mannitol to stabilize 4-aminoantipyrine and acyl coenzyme A activity of oxidase, peroxidase, etc., so as to solve the problem of poor stability and short storage time of the existing single stabilizer kit

However, since the sensitivity of the kit is closely related to the enzyme activity, the kit disclosed in the above report only prolongs the storage time on the basis of ensuring the original activity of the enzyme, and does not substantially improve the sensitivity of the kit.

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