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Preparation method and application of denitrifying bacteria immobilized on graphene-based composite material

A technology of denitrifying bacteria and composite materials, which is applied in the field of preparation of immobilized denitrifying bacteria on graphene-based composite materials, can solve problems such as instability, limited use, and low mechanical strength, and achieve enhanced resistance and increased tolerance , good effect

Inactive Publication Date: 2018-06-01
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common immobilization carriers and embedding materials are polyvinyl alcohol, sodium alginate, agar, gelatin, etc. However, if figure 1 As shown, these immobilized materials have low mechanical strength and are unstable in solutions such as phosphate, which limits their use

Method used

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  • Preparation method and application of denitrifying bacteria immobilized on graphene-based composite material
  • Preparation method and application of denitrifying bacteria immobilized on graphene-based composite material
  • Preparation method and application of denitrifying bacteria immobilized on graphene-based composite material

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Embodiment 1

[0036] A preparation method for graphene-based composite immobilized denitrifying bacteria, the process steps comprising:

[0037] 1) Take two rings of paracocci from the inclined surface and inoculate them into the enrichment medium, culture on a shaking table, centrifuge the culture medium to remove the supernatant, and obtain bacterial cells; suspend the bacterial cells in sterile normal saline, and obtain denitrifying bacteria suspensions. liquid; the concentration of sterile saline is 0.9wt%; the mass ratio of bacteria to sterile saline is 1:30. When the culture medium is cultured on a shaker, the rotation speed is 150r / min, the culture temperature is 27°C, and the culture time is 40h.

[0038] 2) Add 1wt% charcoal powder to the bacterial suspension to absorb for 4 hours to obtain an adsorption solution;

[0039] 3) Prepare graphene-based composite materials by covalent cross-linking method, the specific operation is as follows: add polyvinyl alcohol and sodium alginate ...

Embodiment 2

[0043] A preparation method for graphene-based composite immobilized denitrifying bacteria, the process steps comprising:

[0044] 1) Take two rings of paracocci from the inclined surface and inoculate them into the enrichment medium, culture on a shaking table, centrifuge the culture medium to remove the supernatant, and obtain bacterial cells; suspend the bacterial cells in sterile normal saline, and obtain denitrifying bacteria suspensions. liquid; the concentration of sterile saline is 0.9wt%; the mass ratio of bacteria to sterile saline is 1:10. When the culture medium is cultured on a shaker, the rotation speed is 150r / min, the culture temperature is 30°C, and the culture time is 48h.

[0045] 2) Add 1wt% charcoal powder to the bacterial suspension for adsorption for 6 hours to obtain an adsorption solution;

[0046] 3) Prepare graphene-based composite materials by covalent cross-linking method, the specific operation is as follows: add polyvinyl alcohol and sodium algina...

Embodiment 3

[0050] Application of graphene-based composite material immobilized denitrifying bacteria prepared by the present invention in degrading nitrate nitrogen sewage:

[0051] 1. adopt the method as described in embodiment 1 to prepare graphene-based composite immobilized denitrifying bacteria;

[0052] 2. adjust the artificial sewage nitrate nitrogen content to be 500mg / L, as shown in Table 1, the chemical oxygen demand (abbreviated as COD) pollutant content is 800mg / L, add the prepared graphene-based composite immobilized denitrifying bacteria, add The wet weight is 15g / L, the pH of the solution is 8, and then in a shaker at 180r / min at 30°C until the nitrate nitrogen is fully degraded.

[0053] Table 1 Simulated artificial sewage composition

[0054] Element

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Abstract

The invention discloses a preparation method and application of denitrifying bacteria immobilized on a graphene-based composite material, relating to the field of microorganism environmental protection. The preparation method comprises the steps of (1) inoculating an enrichment medium with denitrifying bacteria to cultivate, centrifuging a cultivation liquid to remove supernate, so as to obtain bacteria; suspending the bacteria in normal saline, so as to obtain a denitrifying bacteria suspension; (2) adsorbing the bacteria suspension for 2-6 hours by using 1wt% of charcoal powder, so as to obtain an adsorption solution; (3) preparing an embedding agent of the graphene-based composite material through a covalent cross-linking method; (4) uniformly mixing the adsorption solution with the embedding agent so as to obtain a mixed carrier; and (5) dripping the mixed carrier into the cross-linking agent drop by drop, cross linking to obtain the immobilized microorganism particles. The prepared immobilized microorganism has high tolerance and high activity, and can treat nitrogen-containing sewage actively and repeatedly.

Description

technical field [0001] The invention relates to the field of microbial environmental protection, in particular to a preparation method and application of a graphene-based composite immobilized denitrifying bacteria. Background technique [0002] Nitrogen is a common pollutant in water bodies, which can lead to eutrophication of water bodies, bring disasters to aquatic organisms, cause human drinking water crisis, endanger human health, and destroy the ecological environment of water bodies. How to economically and efficiently remove nitrogen pollution in water has become a research focus and hotspot in the field of water pollution control. Biological denitrification is the most economical and effective method to remove nitrogen pollution from wastewater, and it can achieve true denitrification. [0003] Biological denitrification technology usually consists of nitrification process and denitrification process. The nitrification process is mainly to remove ammonia nitrogen....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/10C12N11/14C12N11/08C02F3/34C12R1/01C02F101/16
CPCC02F3/34C02F2101/163C12N1/20C12N11/08C12N11/10C12N11/14
Inventor 杨革刘胜格巩志金车程川刘金锋
Owner QUFU NORMAL UNIV
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